Cytotoxicity of Odorous Compounds from Poultry Manure

Dados Bibliográficos

ID15475797
AutoresAdriana Nowak (0000-0002-1865-4833, Lodz University of Technology, autor correspondente), Katarzyna Matusiak (0000-0003-2211-8432, Lodz University of Technology), Sebastian Borowski (0000-0002-8867-3327, Lodz University of Technology), Tadeusz Bakuła (0000-0001-6838-2840, University of Warmia and Mazury in Olsztyn), Sebastian Opaliński (0000-0003-3669-5994, Wrocław University of Environmental and Life Sciences), R Kołacz (0000-0001-7221-5332, Wrocław University of Environmental and Life Sciences), Beata Gutarowska (0000-0002-9523-2001, Lodz University of Technology)
Ano2016
Volume13
Fascículo11
Páginas1046-1046
Data de publicação2016-10-26
Peer ReviewedSim
Open AccessSim
TipoARTICLE
PeriódicoInternational Journal of Environmental Research and Public Health (JOURNAL)
Identificadores do periódicoISSN: 1661-7827 • E-ISSN: 1660-4601
EditoraMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph13111046
PMID27792203
OpenAlexW2542608114
IdiomaEN
Citações recebidas2
Referências citadas22

Long-term exposure and inhalation of odorous compounds from poultry manure can be harmful to farm workers and the surrounding residents as well as animals. The aim of the present study was to determine the cytotoxicity and IC 50 values of common odorous compounds such as ammonium, dimethylamine, trimethylamine, butyric acid, phenol, and indole in the chick liver hepatocellular carcinoma cell line LMH ( Leghorn Male Hepatoma ), in vitro, using MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and PrestoBlue cytotoxicity assays. The cells were microscopically examined for any morphological changes post treatment. Dimethylamine exhibited the strongest cytotoxic effect on LMH cells with an IC 50 value of 0.06% and 0.04% after an exposure of 24 h and 48 h, respectively. Both ammonium and trimethylamine had comparable cytotoxicity and their IC 50 values were 0.08% and 0.04% after 24 h and 48 h, respectively. Of note, indole had the lowest cytotoxicity as the majority of cells were viable even after 72 h exposure. Thus, the IC 50 for indole was not calculated. Results achieved from both MTT and PrestoBlue assays were comparable. Moreover, the morphological changes induced by the tested odours in LMH cells resulted in monolayer destruction, cytoplasm vacuolisation, chromatin condensation, and changes in nucleus and cell shape. Our study showed harmful effects of odorous compounds in chick tissues

Biology · Cytotoxicity · Dimethylamine · IC50 · In vitro · Indole test · Methylamine · MTT assay · Trimethylamine · Advanced Chemical Sensor Technologies · Chemistry · Indoor Air Quality and Microbial Exposure · Odor and Emission Control Technologies · Biochemistry · Molecular Biology · Toxicology