Well preserved non‐collagenous extracellular matrix proteins in ancient human bone and teeth

Datos Bibliográficos

ID12371108
AutoresTyede H Schmidt‐schultz (University of Göttingen, autor de correspondencia), Michael Schultz (0000-0001-5028-2497, University of Göttingen)
Año2006
Volumen17
Número1
Páginas91-99
Fecha de publicación2006-07-13
Peer ReviewedSí
Open AccessSí
TipoARTICLE
RevistaInternational Journal of Osteoarchaeology (JOURNAL)
Identificadores de la revistaISSN: 1047-482X • E-ISSN: 1099-1212
EditorialWiley (PUBLISHER • GB)
DOI10.1002/oa.858
OpenAlexW2031354898
IdiomaEN
Citas recibidas3
Referencias citadas27

Ancient bones in a good preservation state, ascertained by microscopic techniques, conserve extracellular matrix proteins over thousands of years. With new techniques, intact extracellular matrix proteins from ancient bones and teeth are extracted and separated by one‐dimensional and two‐dimensional electrophoresis. Proteins were identified in Western blots by special antibodies against different human extracellular matrix (ECM) molecules of bone. We have confirmed different types of ECM human bone molecules such as osteonectin, osteopontin, and alkaline phosphatase with specific antibodies in human bone samples from different age groups. Additionally, we selected bone samples from different cultural time periods, such as the Middle Ages, the Bronze Age and the Late Pre‐Pottery Neolithic Phase (PPNB), and teeth from individuals from the Early Middle Ages and from the Late PPNB. The survival of intact extracellular matrix proteins in ancient bones and teeth dating from recent times to the Late PPNB, and reliable techniques to identify these proteins, present a great challenge to further research. A Match Set with PD‐Quest 7.2 shows that only 16% of protein spots in the teeth are also found in the bone of the same individual. In combination with the results of macro‐ and microscopic investigation, biochemical techniques will help us in obtaining a better understanding of bone and teeth in health and disease. Copyright © 2006 John Wiley & Sons, Ltd

Alkaline phosphatase · Biology · Blot · Bone matrix · Cartilage · Extracellular · Extracellular matrix · Human bone · Matrix (chemical analysis · Osteocalcin · Osteonectin · Osteopontin · Bone and Dental Protein Studies · Chemistry · Forensic Anthropology and Bioarchaeology Studies · Oral microbiology and periodontitis research · Anatomy · Biochemistry · Cell Biology · Immunology