Reacción en cadena de la polimerasa para la detección rápida y determinación del serotipo de virus del dengue en muestras clínicas
Datos Bibliográficos
| ID | 14968798 |
|---|---|
| Autores | Delfina Rosario (Instituto de Medicina Tropical “Pedro Kourí”, autor de correspondencia), Mayling Álvarez (0000-0002-4718-050X, Instituto de Medicina Tropical “Pedro Kourí”), Javier Díaz (0000-0002-4225-3829, Universidad de Antioquia), Rodolfo Contreras (Instituto de Medicina Tropical “Pedro Kourí”), R Rodríguez (0000-0002-3182-194X, Instituto de Medicina Tropical “Pedro Kourí”), Santiago Vazquez (0000-0002-2707-1031, Instituto de Medicina Tropical “Pedro Kourí”), Susana Vazquez (0000-0002-8367-7513), Maria G Guzmán (0000-0001-6161-9489, Instituto de Medicina Tropical “Pedro Kourí”) |
| Año | 1998 |
| Volumen | 4 |
| Número | 1 |
| Páginas | 1-5 |
| Fecha de publicación | 1998-07-01 |
| Open Access | Sí |
| Tipo | ARTICLE |
| Revista | Revista Panamericana de Salud Pública (JOURNAL) |
| Identificadores de la revista | ISSN: 1020-4989 • E-ISSN: 1680-5348 |
| Editorial | FapUNIFESP (SciELO) (PUBLISHER) |
| DOI | 10.1590/s1020-49891998000700001 |
| PMID | 9734221 |
| OpenAlex | W2051292925 |
| SciELO | S1020-49891998000700001 |
| Idioma | ES |
| Citas recibidas | 1 |
| Referencias citadas | 4 |
This study describes the benefits of using reverse transcriptase polymerase chain reaction (RT-PCR) for the rapid detection and typing of dengue virus in clinical samples. Twenty-seven serum specimens from patients with dengue fever and dengue hemorrhagic fever in Colombia, Nicaragua, and Panama were directly subjected to RT-PCR for the detection of dengue virus. The resulting double-stranded DNA product was typed by a second round of PCR amplification (nested PCR) with type-specific primers, viral culture/indirect immunofluorescence (IIF), and enzyme-linked electroimmunoassay for IgM anti-dengue antibodies. The amplified virus genome was detected and typed within 8 hours. Nested RT-PCR, using viral culture and IIF as the gold standard, showed 100% sensitivity; 78% specificity; 69% positive predictive value, and 100% negative predictive value. It is noteworthy that two of the specimens whose results were positive with nested RT-PCR and negative with viral culture showed specific IgM antibodies. The results of the RT-PCR were in close agreement with those obtained through viral culture. This suggests PCR can greatly facilitate the rapid and early diagnosis of dengue infection
Antibody · Biology · Dengue fever · Dengue virus · Gene · Gold standard (test · IIf · Indirect immunofluorescence · Nested polymerase chain reaction · Polymerase chain reaction · Typing · Viral culture · Virus · Medicine · Mosquito-borne diseases and control · Viral Infections and Outbreaks Research · Viral Infections and Vectors · Immunology · Microbiology · Molecular Biology · Virology
| Citas por año | 0,05 |
|---|---|
| Intervalo de citas | 2006 - 2006 (1) |
| Altamente citado | No |