Brief communication

Multiplex X/Y‐PCR improves sex identification in aDNA analysis

Datos Bibliográficos

ID8313801
AutoresDiane Schmidt (0000-0001-6606-4398, Institute of Zoology, autor de correspondencia), Hummel (0000-0002-3647-8968, Institute of Zoology), Susanne Hummel, Bernd Herrmann (Institute of Zoology)
Año2003
Volumen121
Número4
Páginas337-341
Fecha de publicación2003-08-01
Peer ReviewedSí
Open AccessSí
TipoARTICLE
RevistaAmerican Journal of Physical Anthropology (JOURNAL)
Identificadores de la revistaISSN: 0002-9483 • E-ISSN: 1096-8644
EditorialWiley (PUBLISHER • GB)
DOI10.1002/ajpa.10172
PMID12884315
OpenAlexW2017278739
IdiomaEN
Citas recibidas4
Referencias citadas28

This study introduces a polymerase chain reaction (PCR)‐based multiplex approach to improve the certainty of molecular sex identification on archaeological skeletal material. We coamplified amelogenin, two X‐chromosomal short tandem repeats (STRs) (DXS6789 and DXS9898), and two Y‐specific STRs (DYS391 and DYS392). The amplification results of this multiplex approach back each other up, and enable a reliable sex identification. This coamplification of X‐ and Y‐specific markers in a multiplex assay combines the added advantage of positive identification of both female and male individuals with raising the validity of the diagnosis by obtaining multiple data simultaneously. This multiplex system was successfully applied to 3,000‐year‐old bone material. Am J Phys Anthropol 121:000–000, 2003. © 2003 Wiley‐Liss, Inc

Amelogenin · Biology · Computational biology · Gene · Identification (biology) · Microsatellite · Multiplex · Multiplex polymerase chain reaction · Polymerase chain reaction · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Genetics · Molecular Biology · Molecular Biology Techniques and Applications