Technical note

Improved ancient DNA purification for PCR using ion‐exchange columns

Datos Bibliográficos

ID8315730
AutoresKijeong Kim (0000-0002-5132-1774, Chung-Ang University), Kyung‐yong Kim (0000-0003-2138-4536, Chung-Ang University), Eun-Hee Jeon (Chung-Ang University), Eunhee Jeon, Ariunaa Togloom (Chung-Ang University), Youn‐Ock Cho (Chung-Ang University), Min‐Soo Lee (Chung-Ang University), Gavaachimed Lkhagvasuren (Chung-Ang University), Jee‐Hye Choi (0000-0001-7389-4250, Chung-Ang University), Dashtseveg Tumen (National University of Mongolia), Ae Ja Park (Chung-Ang University), Keun‐Cheol Kim (0000-0003-3047-0380, Kangwon National University), Ki‐Won Park, K-S Park, Jae‐hyun Kim (0000-0002-3531-489X), Jae Hyun Kim (0000-0002-0203-7443, Dong-A University), Maengseok Noh (0000-0002-9713-6144, Pukyong National University), Kwon‐Jong Yoo (Chung-Ang University), Kwang‐Ho Lee (0000-0002-3677-0977, Chung-Ang University, autor de correspondencia)
Año2008
Volumen136
Número1
Páginas114-121
Fecha de publicación2008-05-01
Peer ReviewedSí
Open AccessSí
TipoARTICLE
RevistaAmerican Journal of Physical Anthropology (JOURNAL)
Identificadores de la revistaISSN: 0002-9483 • E-ISSN: 1096-8644
EditorialWiley (PUBLISHER • GB)
DOI10.1002/ajpa.20782
PMID18257014
OpenAlexW2003591562
IdiomaEN
Citas recibidas6
Referencias citadas35

A novel method of ancient DNA (aDNA) purification was developed using ion‐exchange columns to improve PCR‐amplifiable DNA extraction from ancient bone samples. Thirteen PCR‐resistant ancient bone samples aged 500–3,300 years were tested to extract aDNA using a recently reported, silica‐based aDNA extraction method and an ion‐exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high‐copy DNA, and amelogenin, a low‐copy DNA. The results demonstrate that the further purification of silica‐based aDNA extracts using ion‐exchange columns considerably improved PCR amplification. We suggest that the ion‐exchange column‐based method will be useful for the improvement of PCR‐amplifiable aDNA extraction, particularly from the poorly preserved, PCR‐resistant, ancient samples. Am J Phys Anthropol, 2008. © 2008 Wiley‐Liss, Inc

Ancient DNA · Biology · Chromatography · DNA · DNA extraction · Extraction (chemistry) · Gene · Ion · Ion exchange · Polymerase chain reaction · Archaeology and ancient environmental studies · Chemistry · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Genetics · Molecular Biology