Barry Eng
Biographic Data
| ID | 4091480 |
|---|---|
| NAME | Barry Eng |
| GIVEN NAMES | Barry |
| FAMILY NAME | Eng |
| SIGNATURE | ENG B |
| AFFILIATIONS | McMaster University |
| VERIFIED | No |
| TOTAL WORKS | 3 |
| TOTAL CITATIONS | 18 |
| WORKS AS AUTHOR | 3 |
| WORKS AS EDITOR | 0 |
| FIRST PUBLICATION YEAR | 1998 |
| LATEST PUBLICATION YEAR | 2007 |
| H-INDEX | 2 |
Digging deeper into the limits of ancient DNA research on syphilis
Hypersensitive PCR, Ancient Human mtDNA, and Contamination
When highly efficient polymerase was used with high cycle numbers (50-60), strong amplifications were observed, but negative controls were also unexpectedly amplified in a study of ancient human mtDNA from 2000-year-old skeletons. The results of a series of tests revealed that the hypersensitive polymerase chain reaction (PCR) generated by higher cycles and the presence of contaminant DNA (though at extremely low levels) should be responsible for…
Improved DNA extraction from ancient bones using silica-based spin columns
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old
Digging deeper into the limits of ancient DNA research on syphilis
Hypersensitive PCR, Ancient Human mtDNA, and Contamination
When highly efficient polymerase was used with high cycle numbers (50-60), strong amplifications were observed, but negative controls were also unexpectedly amplified in a study of ancient human mtDNA from 2000-year-old skeletons. The results of a series of tests revealed that the hypersensitive polymerase chain reaction (PCR) generated by higher cycles and the presence of contaminant DNA (though at extremely low levels) should be responsible for…
Improved DNA extraction from ancient bones using silica-based spin columns
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old
Hypersensitive PCR, Ancient Human mtDNA, and Contamination
When highly efficient polymerase was used with high cycle numbers (50-60), strong amplifications were observed, but negative controls were also unexpectedly amplified in a study of ancient human mtDNA from 2000-year-old skeletons. The results of a series of tests revealed that the hypersensitive polymerase chain reaction (PCR) generated by higher cycles and the presence of contaminant DNA (though at extremely low levels) should be responsible for…
Digging deeper into the limits of ancient DNA research on syphilis
Ancient DNA (3 works) · Biology (3 works) · Forensic and Genetic Research (3 works) · DNA (2 works) · DNA extraction (2 works) · Gene (2 works) · Medicine (2 works) · Molecular Biology (2 works) · Molecular Biology (2 works) · Polymerase chain reaction (2 works)