Suhua Zhang
Datos Biográficos
| ID | 4357756 |
|---|---|
| NOMBRE | Suhua Zhang |
| NOMBRES | Suhua |
| APELLIDO | Zhang |
| FIRMA | ZHANG S |
| AFILIACIONES | Institute of Forensic Science |
| ORCID | 0000-0002-3984-9728 |
| VERIFICADO | Sí |
| TOTAL DE OBRAS | 18 |
| TOTAL DE CITAS | 6 |
| TOTAL COMO AUTOR | 18 |
| TOTAL COMO EDITOR | 0 |
| PRIMER AÑO DE PUBLICACIÓN | 2017 |
| AÑO MÁS RECIENTE DE PUBLICACIÓN | 2024 |
| ÍNDICE H | 1 |
Y-STR analysis of highly degraded DNA from skeletal remains over 70 years old
The goal of the following study is to clarify whether the skeletal remains over 70 years old from missing persons and their alleged relatives shared identical Y-STR loci. Nowadays, advances in ancient DNA extraction techniques and approaches of using multiple different Y-STRs have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Given the ages and conditions of the skeletal remains, ancient …
Internal validation of an improved system for forensic application
A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application.The multiplex included rapidly mutating Y-STRs and low-medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits.The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases
Different stages of chronic kidney disease are associated with physical performance in adults over 60 years
Objective: The purpose of this study was to determine the association between different stages of chronic kidney disease (CKD) and sarcopenia and its components in the Chinese older population. Methods The study comprised of 2,213 participants aged ≥ 60 years (1,025 men; mean age: 70.7 years) recruited from Shanghai who were invited to participate in a comprehensive geriatric assessment. Sarcopenia was defined according to the AWGS 2019 consensus…
Comparison of three nutritional screening tools for detecting sarcopenia in patients with maintenance hemodialysis
Background: Malnutrition, dynapenia, and sarcopenia are prevalent conditions among patients with maintenance hemodialysis (MHD). They are related to numerous adverse health outcomes. The aim of this study was to compare the effect of three nutritional screening tools on predicting the risk of dynapenia and sarcopenia in patients with MHD. Methods: From July 2020 to April 2021, a total of 849 patients with MHD were enrolled at seven different heal…
Prediction of corneal astigmatism based on corneal tomography after femtosecond laser arcuate keratotomy using a pix2pix conditional generative adversarial network
Purpose: This study aimed to develop a deep learning model to generate a postoperative corneal axial curvature map of femtosecond laser arcuate keratotomy (FLAK) based on corneal tomography using a pix2pix conditional generative adversarial network (pix2pix cGAN) for surgical planning. Methods: A total of 451 eyes of 318 nonconsecutive patients were subjected to FLAK for corneal astigmatism correction during cataract surgery. Paired or single ant…
Investigation on the Genetic-Inconsistent Paternity Cases Using the MiSeq FGx System
Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity ca…
Regulatory Variation within 3’UTR of STAT5A Correlates with Sudden Cardiac Death in Chinese Populations
Definitive diagnosis to sudden cardiac death (SCD) is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death. It is therefore important to uncover the inherited risk component to SCD. Signal transducer and activators of transcription 5 A (STAT5A) is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular …
Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application
Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East A…
Retracted Mitochondrial Genome Sequencing with Short Overlapping Amplicons on Miseq FGx System
With the development and maturation of massively parallel sequencing (MPS) technology, the mitochondrial genome (mitogenome) sequencing is increasingly applied in the forensic field. In this study, we employed the strategy of short overlapping amplicons for the whole mitogenome, library preparation with tagmentation using the Nextera® XT DNA Library Preparation Kit, sequencing on the MiSeq FGx TM Forensic Genomics System and analyzing data using …
Multi-Locus Identification of Psilocybe Cubensis by High-Resolution Melting (HRM)
Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Genetic investigation and phylogenetic analysis of three Chinese ethnic groups using 16 X chromosome STR loci
Background: The value of using X-chromosomal short tandem repeats (X-STRs) as genetic markers in human genetics has been widely recognised. However, the 16 X-STRs in the Goldeneye ® DNA ID System 17X kit have not been thoroughly applied. Aim: To investigate the genetic polymorphisms of 16 X-STRs in three main ethnic minorities (Tibetan, Mongolian and Kazakh) in China and to reveal the phylogenetic relationships of different populations. Subjects …
Genetic polymorphisms of 21 STR loci of Golden e yeTM DNA ID 22NC kit in five ethnic groups of China
Dear Editor, Short tandem repeats (STRs), polymorphic DNA regions with a variable number of repeated units (2–6 base pairs), are attractive to forensic applications such as human identification and
Genetic characterization of four dog breeds with Illumina CanineHD BeadChip
Dear Editor, Due to human's cohabitation with domesticated animals, molecular analysis of animal DNA is increasingly being admitted as evidence in forensic investigations. In 2011, recommendations
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Factors related to HIV infection among unmarried youth in rural areas of Southwest China
We evaluated HIV prevalence among unmarried youth in rural areas of Liangshan Prefecture in southwest China over the period of 2011-2015, and the interactions between behaviors associated with HIV risk. We used HIV sentinel surveillance (HSS) data for our analysis. We used questionnaires to investigate information among unmarried youth aged 15 to 25 years and performed HIV testing. Multivariate logistic regression and path analysis were applied t…
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Massively parallel sequencing of 231 autosomal SNPs with a custom panel
The custom-designed single nucleotide polymorphism (SNP) panel amplified 231 autosomal SNPs in one PCR reaction and subsequently sequenced with massively parallel sequencing (MPS) technology and Ion Torrent personal genome machine (PGM). SNPs were chosen from SNPforID, IISNP, HapMap, dbSNP, and related published literatures. Full concordance was obtained between available MPS calling and Sanger sequencing with 9947A and 9948 controls. Ten SNPs (r…
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Massively parallel sequencing of 231 autosomal SNPs with a custom panel
The custom-designed single nucleotide polymorphism (SNP) panel amplified 231 autosomal SNPs in one PCR reaction and subsequently sequenced with massively parallel sequencing (MPS) technology and Ion Torrent personal genome machine (PGM). SNPs were chosen from SNPforID, IISNP, HapMap, dbSNP, and related published literatures. Full concordance was obtained between available MPS calling and Sanger sequencing with 9947A and 9948 controls. Ten SNPs (r…
Factors related to HIV infection among unmarried youth in rural areas of Southwest China
We evaluated HIV prevalence among unmarried youth in rural areas of Liangshan Prefecture in southwest China over the period of 2011-2015, and the interactions between behaviors associated with HIV risk. We used HIV sentinel surveillance (HSS) data for our analysis. We used questionnaires to investigate information among unmarried youth aged 15 to 25 years and performed HIV testing. Multivariate logistic regression and path analysis were applied t…
Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy…
Genetic polymorphisms of 21 STR loci of Golden e yeTM DNA ID 22NC kit in five ethnic groups of China
Dear Editor, Short tandem repeats (STRs), polymorphic DNA regions with a variable number of repeated units (2–6 base pairs), are attractive to forensic applications such as human identification and
Genetic characterization of four dog breeds with Illumina CanineHD BeadChip
Dear Editor, Due to human's cohabitation with domesticated animals, molecular analysis of animal DNA is increasingly being admitted as evidence in forensic investigations. In 2011, recommendations
Validation of the Investigator 24plex QS Kit
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer m…
Genetic investigation and phylogenetic analysis of three Chinese ethnic groups using 16 X chromosome STR loci
Background: The value of using X-chromosomal short tandem repeats (X-STRs) as genetic markers in human genetics has been widely recognised. However, the 16 X-STRs in the Goldeneye ® DNA ID System 17X kit have not been thoroughly applied. Aim: To investigate the genetic polymorphisms of 16 X-STRs in three main ethnic minorities (Tibetan, Mongolian and Kazakh) in China and to reveal the phylogenetic relationships of different populations. Subjects …
Regulatory Variation within 3’UTR of STAT5A Correlates with Sudden Cardiac Death in Chinese Populations
Definitive diagnosis to sudden cardiac death (SCD) is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death. It is therefore important to uncover the inherited risk component to SCD. Signal transducer and activators of transcription 5 A (STAT5A) is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular …
Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application
Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East A…
Retracted Mitochondrial Genome Sequencing with Short Overlapping Amplicons on Miseq FGx System
With the development and maturation of massively parallel sequencing (MPS) technology, the mitochondrial genome (mitogenome) sequencing is increasingly applied in the forensic field. In this study, we employed the strategy of short overlapping amplicons for the whole mitogenome, library preparation with tagmentation using the Nextera® XT DNA Library Preparation Kit, sequencing on the MiSeq FGx TM Forensic Genomics System and analyzing data using …
Multi-Locus Identification of Psilocybe Cubensis by High-Resolution Melting (HRM)
Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the…
Forensic Parameters of 41 Y-STR Loci in Shandong Han Individuals and Comparison with 42 other Populations
Different stages of chronic kidney disease are associated with physical performance in adults over 60 years
Objective: The purpose of this study was to determine the association between different stages of chronic kidney disease (CKD) and sarcopenia and its components in the Chinese older population. Methods The study comprised of 2,213 participants aged ≥ 60 years (1,025 men; mean age: 70.7 years) recruited from Shanghai who were invited to participate in a comprehensive geriatric assessment. Sarcopenia was defined according to the AWGS 2019 consensus…
Comparison of three nutritional screening tools for detecting sarcopenia in patients with maintenance hemodialysis
Background: Malnutrition, dynapenia, and sarcopenia are prevalent conditions among patients with maintenance hemodialysis (MHD). They are related to numerous adverse health outcomes. The aim of this study was to compare the effect of three nutritional screening tools on predicting the risk of dynapenia and sarcopenia in patients with MHD. Methods: From July 2020 to April 2021, a total of 849 patients with MHD were enrolled at seven different heal…
Prediction of corneal astigmatism based on corneal tomography after femtosecond laser arcuate keratotomy using a pix2pix conditional generative adversarial network
Purpose: This study aimed to develop a deep learning model to generate a postoperative corneal axial curvature map of femtosecond laser arcuate keratotomy (FLAK) based on corneal tomography using a pix2pix conditional generative adversarial network (pix2pix cGAN) for surgical planning. Methods: A total of 451 eyes of 318 nonconsecutive patients were subjected to FLAK for corneal astigmatism correction during cataract surgery. Paired or single ant…
Investigation on the Genetic-Inconsistent Paternity Cases Using the MiSeq FGx System
Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity ca…
Internal validation of an improved system for forensic application
A novel multiplex comprising 41 Y-STR and 3 Y-InDel was developed for forensic application.The multiplex included rapidly mutating Y-STRs and low-medium mutating Y-STRs, which is compatible with many commonly used Y-STR kits.The multiplex is a powerful tool for distinguishing related males, familial searching, and constructing DNA databases
Y-STR analysis of highly degraded DNA from skeletal remains over 70 years old
The goal of the following study is to clarify whether the skeletal remains over 70 years old from missing persons and their alleged relatives shared identical Y-STR loci. Nowadays, advances in ancient DNA extraction techniques and approaches of using multiple different Y-STRs have significantly increased the possibility of obtaining DNA profiles from highly degraded skeletal remains. Given the ages and conditions of the skeletal remains, ancient …
Biology (14 obras) · Genetics (13 obras) · Forensic and Genetic Research (11 obras) · Gene (11 obras) · Genotype (9 obras) · Medicine (8 obras) · Molecular Biology Techniques and Applications (8 obras) · Allele (7 obras) · DNA (6 obras) · Microsatellite (6 obras)