Molecular Probes to Evaluate the Synthesis and Production Potential of an Odorous Compound (2-methylisoborneol) in Cyanobacteria
Dados Bibliográficos
| ID | 15469802 |
|---|---|
| Autores | Keonhee Kim (0000-0002-5725-1447, Konkuk University), Youngdae Yoon (0000-0002-7456-0348, Konkuk University), Hyukjin Cho (Korea Fisheries Resources Agency), Soon‐Jin Hwang (0000-0001-7083-5036, Konkuk University, autor correspondente) |
| Ano | 2020 |
| Volume | 17 |
| Fascículo | 6 |
| Páginas | 1933-1933 |
| Data de publicação | 2020-03-16 |
| Peer Reviewed | Sim |
| Open Access | Sim |
| Tipo | ARTICLE |
| Periódico | International Journal of Environmental Research and Public Health (JOURNAL) |
| Identificadores do periódico | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Editora | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph17061933 |
| PMID | 32188031 |
| OpenAlex | W3010898171 |
| Idioma | EN |
| Referências citadas | 32 |
The volatile metabolite, 2-Methylisoborneol (2-MIB) produced by cyanobacterial species, causes odor and taste problems in freshwater systems. However, simple identification of cyanobacteria that produce such off-flavors may be insufficient to establish the causal agent of off-flavor-related problems as the production-related genes are often strain-specific. Here, we designed a set of primers for detecting and quantifying 2-MIB-synthesizing cyanobacteria based on mibC gene sequences (encoding 2-MIB synthesis-catalyzing monoterpene cyclase) from various Oscillatoriales and Synechococcales cyanobacterial strains deposited in GenBank. Cyanobacterial cells and environmental DNA and RNA were collected from both the water column and sediment of a eutrophic stream (the Gong-ji Stream, Chuncheon, South Korea), which has a high 2-MIB concentration. Primer sets mibC196 and mibC300 showed universality to mibC in the Synechococcales and Oscillatoriales strains; the mibC132 primer showed high specificity for Pseudanabaena and Planktothricoides mibC . Our mibC primers showed excellent amplification efficiency (100-102%) and high correlation among related variables (2-MIB concentration with water RNA r = 689, p p p p mibC genes (and thus 2-MIB-producing potential) and for evaluating mibC gene expression as an early warning of massive cyanobacterial occurrence
Bacteria · Biology · Botany · Cyanobacteria · DNA · GenBank · Gene · Microcystis · Microcystis aeruginosa · Primer (cosmetics · RNA · Algal biology and biofuel production · Aquatic Ecosystems and Phytoplankton Dynamics · Biocrusts and Microbial Ecology · Chemistry · Biochemistry · Genetics
| Velocidade de citação | historical |
|---|---|
| Altamente citado | Não |