Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections

Dados Bibliográficos

ID15480486
AutoresHanoch Martins (Universidade Federal de Minas Gerais), Maria Rosa Quaresma Bomfim (0000-0001-5738-3952, Universidade Federal de Minas Gerais), Maria Bomfim (Universidade Federal de Minas Gerais), Rafaela Oliveira França (Universidade Federal de Minas Gerais), Rafaela FRANCA (0009-0006-5581-0512, Universidade Federal de Minas Gerais), Luiz de Mácêdo Farias (0000-0002-7462-767X, Universidade Federal de Minas Gerais), Luiz Farias (Universidade Federal de Minas Gerais), Maria Carvalho (0000-0002-2571-5868, Universidade Federal de Minas Gerais), Maria de Lourdes Ribeiro de Carvalho (Universidade Federal de Minas Gerais), José Carlos Serufo (Universidade Federal de Minas Gerais), José Serufo (Universidade Federal de Minas Gerais), Simone Santos (0000-0002-2757-4480, Universidade Federal de Minas Gerais), Simone Gonçalves dos Santos (0000-0003-3777-853X, Universidade Federal de Minas Gerais, autor correspondente)
Ano2014
Volume11
Fascículo2
Páginas1465-1478
Data de publicação2014-01-28
Peer ReviewedSim
Open AccessSim
TipoARTICLE
PeriódicoInternational Journal of Environmental Research and Public Health (JOURNAL)
Identificadores do periódicoISSN: 1661-7827 • E-ISSN: 1660-4601
EditoraMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph110201465
PMID24477210
PMCIDPMC3945548
OpenAlexW2118059978
IdiomaEN
Referências citadas26

In this study, phenotypic and genotypic methods were used to detect metallo-β-lactamases, cephalosporinases and oxacillinases and to assess genetic diversity among 64 multiresistant Acinetobacter baumannii strains recovered from blood cultures in five different hospitals in Brazil from December 2008 to June 2009. High rates of resistance to imipenem (93.75%) and polymyxin B (39.06%) were observed using the disk diffusion (DD) method and by determining the minimum inhibitory concentration (MIC). Using the disk approximation method, thirty-nine strains (60.9%) were phenotypically positive for class D enzymes, and 51 strains (79.6%) were positive for cephalosporinase (AmpC). Using the E-test, 60 strains (93.75%) were positive for metallo-β-lactamases (MβLs). All strains were positive for at least one of the 10 studied genes; 59 (92.1%) contained blaVIM-1, 79.6% contained blaAmpC, 93.7% contained blaOXA23 and 84.3% contained blaOXA51. Enterobacteria Repetitive Intergenic Consensus (ERIC)-PCR analysis revealed a predominance of certain clones that differed from each other. However, the same band pattern was observed in samples from the different hospitals studied, demonstrating correlation between the genotypic and phenotypic results. Thus, ERIC-PCR is an appropriate method for rapidly clustering genetically related isolates. These results suggest that defined clonal clusters are circulating within the studied hospitals. These results also show that the prevalence of MDR A. baumannii may vary among clones disseminated in specific hospitals, and they emphasize the importance of adhering to appropriate infection control measures

Acinetobacter · Acinetobacter baumannii · Antibiotic resistance · Antibiotics · Bacteria · Biology · Gene · Genetic diversity · Genome · Genotype · Imipenem · Intergenic region · Polymerase chain reaction · Polymyxin · Pseudomonas aeruginosa · Antibiotic Resistance in Bacteria · Medicine · Pharmaceutical and Antibiotic Environmental Impacts · Vibrio bacteria research studies · Genetics · Microbiology