Resistoma y genómica comparativa de aislados clínicos de Escherichia coli diarreogénica en Lima, Perú

Dados Bibliográficos

ID15534872
AutoresWilli Quino (0000-0002-3693-1531, Instituto Nacional de Salud, autor correspondente), Orson Mestanza (0000-0001-7268-0496, Instituto Nacional de Salud), Junior Caro-Castro (0000-0002-8890-6119, Instituto Nacional de Salud), Carmen V Hurtado (0000-0003-3667-6806, Instituto Nacional de Salud), Ronnie G Gavilán (0000-0003-1437-5607, Universidad Privada San Juan Bautista)
Ano2020
Volume37
Fascículo4
Páginas705-10
Data de publicação2020-11-18
Peer ReviewedSim
Open AccessSim
TipoARTICLE
PeriódicoRevista Peruana de Medicina Experimental y Salud Pública (JOURNAL)
Identificadores do periódicoISSN: 1726-4634 • E-ISSN: 1726-4642
EditoraNational Institute of Health of Peru (PUBLISHER • PE)
DOI10.17843/rpmesp.2020.374.5240
PMID33566911
OpenAlexW3108160211
IdiomaES
Referências citadas2

In order to describe the genomic characteristics related to antimicrobial resistance and comparative genomics of diarrheagenic Escherichia coli (DEC), 14 DEC isolates from the strain collection of the Instituto Nacional de Salud (INS) were subjected to genome sequencing. We used bioinformatic procedures to analyze the obtained sequences in order to look for microbial resistance genes and genetic regions related to pathotypes and phylogroups. Several antimicrobial resistance determinants were detected, but the production of beta-lactamases and mutations associated to quinolone resistance were the most relevant. Additionally, we observed isolates of the same pathotype grouped in different phylogroups. The comparative genomics analysis showed a greater number of orthologous genes in isolates from the same pathotype and phylogroup. In conclusion, DEC isolates from Lima, Peru, showed resistance to multiple drugs; likewise, molecular and phylogenetic diversity was observed in several pathotypes and phylogroups

Biology · Humanities · Philosophy · vaccines and immunoinformatics approaches · Molecular Biology