Sex estimation of teeth at different developmental stages using dimorphic enamel peptide analysis

Dados Bibliográficos

ID8317683
AutoresRebecca L Gowland (0000-0002-5909-6460, Department of Archaeology Durham University Durham UK, autor correspondente), Nicolas A Stewart (0000-0002-8969-4242, School of Pharmacy and Biomolecular Sciences University of Brighton Brighton UK), Kyle D Crowder (0000-0002-0861-6489, Durham University), Kayla D Crowder (Department of Archaeology Durham University Durham UK), Claire M Hodson (0000-0002-3408-2871, Durham University), Claire Hodson (Department of Archaeology Durham University Durham UK), Heidi Shaw (Department of Archaeology Durham University Durham UK), K J Gron (0000-0002-0415-2891, Durham University), J Montgomery (0000-0001-6729-3088, Durham University)
Ano2021
Volume174
Fascículo4
Páginas859-869
Data de publicação2021-04-01
Peer ReviewedSim
Open AccessSim
TipoARTICLE
PeriódicoAmerican Journal of Physical Anthropology (JOURNAL)
Identificadores do periódicoISSN: 0002-9483 • E-ISSN: 1096-8644
EditoraWiley (PUBLISHER • GB)
DOI10.1002/ajpa.24231
PMID33475153
OpenAlexW3122655611
IdiomaEN
Citações recebidas23
Referências citadas23

OBJECTIVES: This study tests, for the first time, the applicability of a new method of sex estimation utilizing enamel peptides on a sample of deciduous and permanent teeth at different stages of mineralization, from nonadults of unknown sex, including perinates. MATERIALS AND METHODS: A total of 43 teeth from 29 nonadult individuals aged from 40 gestational weeks to 19 years old were analyzed. The sample included pairs of fully mineralized and just developing teeth from the same individual. The individuals were from four archaeological sites in England: Piddington (1st-2nd centuries AD), Coach Lane, Victoria Gate, and Fewston (all 18th-19th centuries). A method that identifies sex chromosome-linked isoforms of the peptide amelogenin from human tooth enamel was applied. The method utilizes a minimally destructive acid etching procedure and subsequent nano liquid chromatography tandem mass spectrometry. RESULTS: It was possible to determine the sex of 28 of the nonadult individuals sampled (males = 20, females = 8, undetermined = 1). Only one sample failed (CL9), due to insufficient mineralization of the sampled tooth enamel. Data are available via ProteomeXchange with identifier PXD021683. DISCUSSION: Sufficient peptide material to determine sex can be recovered even from the crowns of developing perinatal teeth that are not fully mineralized. The minimally destructive and inexpensive (compared to ancient DNA) nature of this procedure has significant implications for bioarchaeological studies of infancy and childhood

Amelogenin · Biology · Deciduous teeth · Dental enamel · Enamel paint · Permanent teeth · Sexual dimorphism · Zoology · Bone and Dental Protein Studies · Dentistry · Forensic and Genetic Research · Forensic Anthropology and Bioarchaeology Studies · Medicine · Orthodontics