Modernisms

Background: Candida species are common organisms in human and animal mucosa that cause a wide range of \nCandida infections in immunocompromised patients. This study investigated the ability to produce proteinase, \nphospholipase and hemolysin as well as biofilm formation in different clinical isolates of Candida albicans. \nMethods: In this study, ninety-four C. albicans were identified using phenotypic tests and amplification of the \nhyphal wall protein (HWP1) gene, and the proteinase, phospholipase and hemolysin production in specific \nmediums, as well as the ability to biofilm formation using the crystal violet method were evaluated. Then, the \nantifungal susceptibility of planktonic cells was tested on the basis of the CLSI- M27-A3/S protocol. \nFindings: In this study, the proteinase, phospholipase and hemolysin activities of C.albicans isolated from \ndifferent body sites were 82%, 75.5%, and 68%, respectively. Additionally, 74.5% of the isolates had the ability \nto biofilm formation. Among the isolates being studied, the strains isolated from the oral cavity showed the \nhighest activity of proteinase, hemolysin and biofilm formation, and the strains isolated from vaginal secretions \nshowed the highest level of phospholipase activity. The susceptibility pattern of C. albicans species to \nantifungals showed that all isolates were sensitive to AMB and VRC, and resistance to FLC and ITC was \nreported as 5.4% and 2.2%, respectively. \nConclusion: The results show the importance of molecular epidemiology studies and understanding the role of \nhydrolytic enzymes and biofilm production in C. albicans strains. \nKeywords: Candida albicans; Antifungal agents; Disease susceptibility; Virulence; Biofilms

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