Estimation of the age of human semen stains by attenuated total reflection Fourier transform infrared spectroscopy
A preliminary study
Bibliographic Data
| ID | 13119613 |
|---|---|
| Authors | Shuai Zha (0000-0003-3636-1177, Xi'an Jiaotong University), Xin Wei (0000-0003-0976-3381, Xi'an Jiaotong University), Ruoxi Fang (Xi'an Jiaotong University), Qi Wang (0000-0003-0464-2118, Chongqing Medical University), Hancheng Lin (Xi'an Jiaotong University), Kai Zhang (0000-0002-7401-2931, Xi'an Jiaotong University), Haohui Zhang (0000-0001-8432-3672, Xi'an Jiaotong University), Ruina Liu (0000-0003-2671-4266, Xi'an Jiaotong University), Zhouru Li (0009-0001-5832-2534, Xuzhou Medical College), Ping Huang (0000-0002-7573-715X, Institute of Forensic Science, corresponding author), Zhenyuan Wang (0000-0002-5875-1355, Xi'an Jiaotong University, corresponding author) |
| Year | 2019 |
| Volume | 5 |
| Issue | 2 |
| Pages | 119-125 |
| Publication date | 2019-09-09 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Forensic Sciences Research (JOURNAL) |
| Journal identifiers | ISSN: 2471-1411 • E-ISSN: 2096-1790 |
| Publisher | Taylor & Francis (PUBLISHER • GB) |
| DOI | 10.1080/20961790.2019.1642567 |
| PMID | 32939428 |
| OpenAlex | W2973028573 |
| Language | EN |
| References cited | 27 |
Semen stain is one of the most important biological evidence at sexual crime scenes. Age estimation of human semen stains plays an important role in forensic work, and it is rarely studied due to lack of well-established methods. In this study, the technique called attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) coupled with advanced chemometric methods was employed to determine the age of semen stains on three different substrates: glass slides, tissues and fabric made of regenerated cellulose fibres up to 6 d. Partial least squares regression (PLSR) was used in conjunction with spectral analysis for age estimation, and the results generated high R 2 values (cross-validation: 0.81, external validation: 0.74) but a narrow margin of error for root mean square error (RMSE) (RMSE of cross-validation: 0.77 d, RMSE of prediction: 1.02 d). Additionally, our results indicated the robustness of PLSR model was not weaken by the influence of different substrates in this study. Our results indicate that ATR-FTIR, combined with chemometric methods, shows great potential as a convenient and efficient tool for age estimation of semen stains. Moreover, the method could be applied to routine forensic investigations in the future
Analytical Chemistry (journal · Attenuated total reflection · Biology · Chemometrics · Chromatography · Fourier transform · Fourier transform infrared spectroscopy · Mean squared error · Optics · Partial least squares regression · Physics · Semen · Statistics · Bee Products Chemical Analysis · Chemistry · Mathematics · Spectroscopy and Chemometric Analyses · Spectroscopy Techniques in Biomedical and Chemical Research · Anatomy
| Highly cited | No |
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