Descriptive analysis of protein expression variations during pupal development of Chrysomya megacephala (Diptera
(Calliphoridae) using label-free proteomic techniques
Bibliographic Data
| ID | 13119784 |
|---|---|
| Authors | Ren Long (0000-0002-4150-8682, Hainan Medical University, corresponding author), C N Luo (0000-0003-3471-4442, Institute of Forensic Science), Chengyong Luo (Guangdong Nantian Institute of Forensic Science , Shenzhen), Peng Zhang (0000-0001-6953-800X, Hainan Medical University), Xing Zou (0009-0006-8632-1770, Hainan Medical University), Tao Song (0000-0002-5965-3140, Hainan Medical University) |
| Year | 2025 |
| Volume | 10 |
| Issue | 3 |
| Pages | owaf003-owaf003 |
| Publication date | 2025-01-16 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Forensic Sciences Research (JOURNAL) |
| Journal identifiers | ISSN: 2471-1411 • E-ISSN: 2096-1790 |
| Publisher | Taylor & Francis (PUBLISHER • GB) |
| DOI | 10.1093/fsr/owaf003 |
| PMID | 40567407 |
| OpenAlex | W4406440068 |
| Language | EN |
| References cited | 41 |
Age estimation is a critical aspect of forensic entomology, especially in the examination of pupae. The use of proteins as a means for age identification shows great promise. In this study, proteomic techniques were employed to investigate differentially expressed proteins (DEPs) during the intrapuparial stage of Chrysomya megacephala . Specimens were sampled at four distinct time points: 0 h (Group A), 24 h (Group B), 48 h (Group C), and 72 h (Group D). Our analysis uncovered 56 DEPs between Groups B and A, 116 DEPs between Groups C and A, and a total of 152 DEPs between Groups D and A. These DEPs were categorized into nine clusters based on their expression patterns. Cluster 1 exhibited an increasing trend in protein expression, while Cluster 4 displayed the opposite pattern. Clusters 2, 6, and 9 showed an initial rise followed by a decline, whereas Cluster 3 demonstrated the reverse trend. Cluster 8 indicated an initial rise, a subsequent drop, and another rise, while Cluster 7 showed an initial decrease, followed by an increase and a minor decrease. Notably, the C-type lectin domain-containing (CTLD) protein and Failed axon connections (Fax) protein consistently displayed an upward trend. These two DEPs were selected for validation using the parallel reaction monitoring technique-targeted proteomics, confirming the trends observed in the initial analysis. In summary, this study highlights the potential of using proteins as reliable biomarkers for estimating pupal age
Biology · Calliphoridae · Chrysomya megacephala · Forensic Entomology · Larva · Pupa · Zoology · Forensic Entomology and Diptera Studies · Insect and Pesticide Research · Insect Utilization and Effects · Ecology
| Highly cited | No |
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