Characterizing the Interaction between Antibiotics and Humic Acid by Fluorescence Quenching Method
Bibliographic Data
| ID | 15464302 |
|---|---|
| Authors | Runze Wang (0000-0003-2992-6352, Chang'an University), Shengke Yang (0000-0002-0115-543X, Chang'an University, corresponding author), Jie Fang (0000-0003-2545-604X, Chang'an University), Zongzhou Wang (0000-0002-5802-7121, Chang'an University), Yangyang Chen (0009-0006-3864-1040, Chang'an University), Dan Zhang (0000-0001-6554-7283, Chang'an University), Chunyan Yang (0000-0002-4536-6378, Chang'an University) |
| Year | 2018 |
| Volume | 15 |
| Issue | 7 |
| Pages | 1458-1458 |
| Publication date | 2018-07-10 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph15071458 |
| PMID | 29996545 |
| OpenAlex | W2878438014 |
| Language | EN |
| Citations received | 2 |
| References cited | 41 |
The method of Three-Dimensional Excitation Emission Matrix Fluorescence Spectrophotometer was used to identify the interaction mechanism between humic acid (HA) and antibiotics. The effects of antibiotic concentration, temperature, and pH on the bonding strength between HA and antibiotics were investigated. The results showed that with the increase of antibiotics concentration, the quenching effect on HA was enhanced. The quenching of HA by both oxytetracycline (OTC) and sulfadiazine (SD) is a single static quenching of the complexes, and the interaction forces are mainly a hydrogen bond and the van der Waals force. The quenching constant K sv OTC > K sv SD and the binding constant K b OTC > K b SD, indicates that HA has a more obvious quenching effect on OTC. The K b of HA by OTC ranged from 3.223 × 103 to 12.784 × 103 L·mol −1 in the range of 298 K to 313 K, while the K b of HA by SD ranged from 2.051 × 103 to 5.533 × 103 L·mol −1 . With the increase of temperature, the quenching constant K sv and binding constant K b of both OTC and SD by HA gradually decrease, and the low temperature is beneficial to the interaction. The composite of OTC and HA is more stable than SD. Under neutral alkaline conditions, both OTC and SD had the strongest quenching effect on HA, and the resulting complex was the most stable. However, the K sv and Kb of HA by OTC were greater than SD in the pH range of the experiment and the pH effect on quenching of HA by OTC ( K sv ) was greater than that of SD
Antibiotics · Fluorescence · Humic acid · Optics · Photochemistry · Physics · Quenching (fluorescence · Chemistry · Pesticide and Herbicide Environmental Studies · Pesticide Residue Analysis and Safety · Pharmaceutical and Antibiotic Environmental Impacts · Biochemistry · Environmental Chemistry · Organic Chemistry
| Unique citing works | 2 |
|---|---|
| Citations per year | 0,25 |
| Citation span | 2018 - 2020 (3) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 2 |