Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy
The Respira Study
Bibliographic Data
| ID | 15466355 |
|---|---|
| Authors | Claudia Zani (0000-0002-6454-597X, University of Brescia), Elisabetta Ceretti (0000-0002-4856-5364, University of Brescia), Ilaria Zerbini (0000-0002-9337-2166, University of Brescia), Gaia Claudia Viviana Viola (0000-0002-6904-7232, University of Brescia), Francesco Donato (0000-0002-1402-1019, University of Brescia), Umberto Gelatti (0000-0001-7888-5716, University of Brescia), Donatella Feretti (0000-0002-8455-6174, University of Brescia, corresponding author) |
| Year | 2020 |
| Volume | 17 |
| Issue | 9 |
| Pages | 3276-3276 |
| Publication date | 2020-05-08 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph17093276 |
| PMID | 32397090 |
| OpenAlex | W3023271861 |
| Language | EN |
| Citations received | 2 |
| References cited | 49 |
Air pollution is a well-known problem for human health, especially for children living in highly polluted urban areas. This study aimed to assess the relationship between airborne pollutants concentration and biomarkers of DNA damage in the buccal mucosa cells of pre-school children. DNA damage was investigated with comet test in saliva leukocytes taken from sputum of 3- to 6-year-old children living in Brescia, Northern Italy, collected during two consecutive winter seasons (2012-2013). The daily levels of PM10, PM2.5, NO 2 , CO, SO 2 , benzene and O 3 in urban air were collected for the whole period. A questionnaire filled in by the children's parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children's buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO 2
Air pollutants · Air pollution · Biology · Buccal swab · Comet Assay · DNA · DNA damage · Environmental health · Pathology · Pollutant · Saliva · Sputum · Air Quality and Health Impacts · Carcinogens and Genotoxicity Assessment · Environmental Science · Medicine · Noise Effects and Management · Ecology · Genetics · Internal Medicine · Molecular Biology · Physiology · Toxicology
| Unique citing works | 2 |
|---|---|
| Citations per year | 0,33 |
| Citation span | 2020 - 2021 (2) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 2 |