Investigating the Antioxidant and Cytocompatibility of Mimusops elengi Linn Extract over Human Gingival Fibroblast Cells

Bibliographic Data

ID15466912
AuthorsShaeesta Khaleelahmed Bhavikatti (0000-0001-6332-5492, Universiti Sains Malaysia, corresponding author), Mohmed Isaqali Karobari (0000-0002-0313-9695, Universiti Sains Malaysia), Siti Lailatul Akmar Zainuddin (0000-0002-2485-1251, Universiti Sains Malaysia), Anand Marya (0000-0003-2009-4393, University of Puthisastra), Sameer Nadaf (0000-0002-7132-1886), V J Sawant (0000-0002-3350-7049), Sandeep B Patil (0000-0002-1697-3267), Adith Venugopal (0000-0002-7945-8799, Saveetha University), Pietro Messina (0000-0001-7409-4474, University of Palermo), Giuseppe Alessandro Scardina (0000-0001-7305-530X, University of Palermo, corresponding author)
Year2021
Volume18
Issue13
Pages7162-7162
Publication date2021-07-04
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph18137162
PMID34281099
OpenAlexW3182912889
LanguageEN
Citations received15
References cited1

Background-chlorhexidine (CHX) is most commonly used as a chemical plaque control agent. Nevertheless, its adverse effects, including teeth discoloration, taste alteration and calculus build-up, limit its use and divert us to medicinal herbs. The purpose of the study was to evaluate the phytochemical composition, antioxidant potential, and cytotoxic effects of Mimusops elengi Linn extract (ME) over normal human cultured adult gingival fibroblasts (HGFs). Methods-in vitro phytochemical screening, total flavonoid content, antioxidant potential by DPPH and Nitric Oxide (NO) radical scavenging activity, and cytotoxic effects of ME extracts over HGF were explored. The viability of HGF cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), neutral red uptake, and trypan blue assay after treatment with different concentrations of CHX and ME (0.3125 to 10 μg/mL). Results - ME showed some alkaloids, glycosides, saponins and flavonoids exhibited relatively moderate-to-good antioxidant potential. Increasing the concentration of CHX and ME from 0.3125 to 10 μg/mL reduced cell viability from 29.71% to 1.07% and 96.12% to 56.02%, respectively. At higher concentrations, CHX reduced the viability of cells by 52.36-fold compared to ME, revealed by MTT assay. At 10 μg/mL concentration, the mean cell viability of CHX and ME-treated cells was 2.24% and 57.45%, respectively, revealed by a neutral red assay. The viability of CHX- and ME-treated HGF cells estimated at higher concentrations (10 μg/mL) using trypan blue assay was found to be 2.18% and 47.36%, respectively. A paired t -test showed significance ( p < 0.05), and one-way ANOVA difference between the mean cell viability of CHX- and ME-treated cells at different concentrations. One-way ANOVA confirmed the significant difference between the viability of CHX- and ME-treated cells. Conclusions-The cytoprotective and antioxidant effects of ME emphasize its potential benefits. Therefore, it could emerge as a herbal alternative and adjunct to conventional oral hygiene methods, that can diminish periodontal tissue destruction

Antioxidant · Chlorhexidine · Cytotoxic T cell · Fibroblast · Phytochemical · Taste · Traditional medicine · Chemistry · Curcumin's Biomedical Applications · Dentistry · Ginger and Zingiberaceae research · Inflammatory mediators and NSAID effects · Medicine · Biochemistry · Food Science

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