Safety and Protective Effectiveness of Two Strains of Lactobacillus with Probiotic Features in an Experimental Model of Salmonellosis

Bibliographic Data

ID15467850
AuthorsRaphael da Silva Steinberg (Universidade Federal de Minas Gerais, corresponding author), Raphael Steinberg (Universidade Federal de Minas Gerais), Lílian Silva (0009-0007-3913-2659, Universidade Federal de Minas Gerais), Lilian C Silva e Silva (Universidade Federal de Minas Gerais), Tassia Costa Souza (Universidade Federal de Minas Gerais), Tássia Souza (Universidade Federal de Minas Gerais), Maurício Lima (0000-0002-3700-2945, Universidade Federal de Minas Gerais), Nayara De Oliveira (Universidade Federal de Minas Gerais), Leda Quercia VIEIRA (0000-0001-5481-9618, Universidade Federal de Minas Gerais), Leda Vieira (Universidade Federal de Minas Gerais), Rosa Maria Esteves Arantes (0000-0003-1428-9717, Universidade Federal de Minas Gerais), Rosa Arantes (Universidade Federal de Minas Gerais), Anderson Miyoshi (0000-0002-0480-8993, Universidade Federal de Minas Gerais), Jacques R Nicoli (0000-0003-2390-2608, Universidade Federal de Minas Gerais), Jacques Nicoli (Universidade Federal de Minas Gerais), Elisabeth Neumann (Universidade Federal de Minas Gerais), Álvaro Cantini Nunes (0000-0001-8844-8333, Universidade Federal de Minas Gerais), Álvaro Nunes (Universidade Federal de Minas Gerais)
Year2014
Volume11
Issue9
Pages8755-8776
Publication date2014-08-26
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph110908755
PMID25162711
PMCIDPMC4198989
OpenAlexW2060502769
LanguageEN
Citations received1
References cited57

Two strains of Lactobacillus, previously isolated from bovine faeces and tested in vitro for properties desired in probiotics, were evaluated for their in vivo effectiveness in protecting against experimental salmonellosis. L. salivarius L38 and L. acidophilus L36 previously demonstrated the ability to successfully colonize the gastrointestinal tract of germ-free mice and stimulate the immune system associated with the intestinal mucosa. L38- or L36-feeding showed no detrimental effect on the general health indicators and did not induce changes in normal architecture of liver and small intestine, indicating that the use of these strains is apparently safe. In control animals fed L38 strain, several cytokines had augmented mRNA levels that can be associated with a homeostatic state of intestinal mucosa, while L36 had less diverse regulation. IgA production and secretion in the intestinal lumen induced by infection was abrogated by pretreating with both lactobacilli. In addition, liver and small intestine histological scores and, translocation of Salmonella cells to liver and spleen, indicated that these strains did not confer protection against the infection. So, the IL-12:IL-18àIFN-g axis, essential for an effective immune response against Salmonella, was not favored with L38 or L36 strains. However, increased expression of IL-10 in different portions of the gastrointestinal tract of L38-fed animals is indicative of anti-inflammatory effect to be explored furthermore

Bacteria · Biology · Gastrointestinal tract · Immune system · In vivo · Lactobacillus · Lactobacillus acidophilus · Lactobacillus salivarius · Probiotic · Salmonella · Spleen · Gut microbiota and health · Probiotics and Fermented Foods · Viral gastroenteritis research and epidemiology · Biotechnology · Immunology · Microbiology

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