Three Experimental Common High-Risk Procedures
Emission Characteristics Identification and Source Intensity Estimation in Biosafety Laboratory
Bibliographic Data
| ID | 15468653 |
|---|---|
| Authors | Zhijian Liu (0000-0003-1841-2671, North China Electric Power University, corresponding author), Jiabin Lv (North China Electric Power University), Zheng Zhang (0000-0002-0389-1844, North China Electric Power University), Juntao Ma (0000-0002-0984-5910, North China Electric Power University), Yangfan Song (North China Electric Power University), Minnan Wu (0000-0003-3322-3115, North China Electric Power University), Guoqing Cao (0000-0001-9124-8914, China Academy of Building Research), Jiacheng Guo (0000-0003-4742-9907, North China Electric Power University) |
| Year | 2023 |
| Volume | 20 |
| Issue | 5 |
| Pages | 4479-4479 |
| Publication date | 2023-03-02 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph20054479 |
| PMID | 36901493 |
| OpenAlex | W4323044229 |
| Language | EN |
| References cited | 45 |
Biosafety laboratory is an important place to study high-risk microbes. In biosafety laboratories, with the outbreak of infectious diseases such as COVID-19, experimental activities have become increasingly frequent, and the risk of exposure to bioaerosols has increased. To explore the exposure risk of biosafety laboratories, the intensity and emission characteristics of laboratory risk factors were investigated. In this study, high-risk microbe samples were substituted with Serratia marcescens as the model bacteria. The resulting concentration and particle size segregation of the bioaerosol produced by three experimental procedures (spill, injection, and sample drop) were monitored, and the emission sources' intensity were quantitatively analyzed. The results showed that the aerosol concentration produced by injection and sample drop was 103 CFU/m3, and that by sample spill was 102 CFU/m3. The particle size of bioaerosol is mainly segregated in the range of 3.3-4.7 μm. There are significant differences in the influence of risk factors on source intensity. The intensity of sample spill, injection, and sample drop source is 3.6 CFU/s, 78.2 CFU/s, and 664 CFU/s. This study could provide suggestions for risk assessment of experimental operation procedures and experimental personnel protection
Aerosol · Aerosolization · Bioaerosol · Biology · Biosafety · Chromatography · Indoor bioaerosol · Respirator · Risk assessment · Sample (material · Air Quality and Health Impacts · Chemistry · Computer Science · Environmental Science · Indoor Air Quality and Microbial Exposure · Infection Control and Ventilation · Materials Science · Medicine · Biotechnology · Environmental Chemistry · Toxicology
| Highly cited | No |
|---|