Comparison of Culture- and Quantitative PCR-Based Indicators of Antibiotic Resistance in Wastewater, Recycled Water, and Tap Water
Bibliographic Data
| ID | 15469228 |
|---|---|
| Authors | Jaqueline Rocha (0000-0003-1651-9483, Universidade Católica Portuguesa), Telma Fernandes (0000-0002-5954-5609, Universidade Católica Portuguesa), Maria V Riquelme (0000-0003-1142-109X, Virginia Tech), Ni Zhu (0000-0003-3294-5167, Virginia Tech), Amy Pruden (0000-0002-3191-6244, Virginia Tech, corresponding author), Célia M Manaia (0000-0002-3674-1789, Universidade Católica Portuguesa, corresponding author) |
| Year | 2019 |
| Volume | 16 |
| Issue | 21 |
| Pages | 4217-4217 |
| Publication date | 2019-10-30 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph16214217 |
| PMID | 31671709 |
| OpenAlex | W2982708289 |
| Language | EN |
| Citations received | 1 |
Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation bla OXA-1 gene (0%-57%). Presumptive total and fecal coliforms and cefotaxime-resistant total coliforms strongly correlated with bla CTX-M and intI 1 (0.725 ≤ R 2 ≤ 0.762; p Escherichia coli -specific biomarkers, gadAB, and uidA , suggesting that both methods captured fecal-sourced bacteria. The genes encoding resistance to sulphonamides ( sul1 and sul2 ) were the most abundant, followed by genes encoding resistance to tetracyclines ( tet (A) and tet (O)) and β-lactams ( bla OXA-1 and , bla CTX-M ), which was in agreement with the culture-based enumerations. The findings can help inform future application of methods being considered for international antibiotic resistance surveillance in the environment
Antibiotic resistance · Antibiotics · Bacteria · Biology · Cefotaxime · Drug resistance · Escherichia coli · Fecal coliform · Feces · Gene · Indicator bacteria · Tap water · Tetracycline · Veterinary medicine · Wastewater · Water quality · Antibiotic Resistance in Bacteria · Antibiotic Use and Resistance · Environmental Science · Medicine · Pharmaceutical and Antibiotic Environmental Impacts · Ecology · Environmental Engineering · Genetics · Microbiology
| Unique citing works | 1 |
|---|---|
| Citations per year | 0,17 |
| Citation span | 2020 - 2020 (1) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 1 |