Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes
Bibliographic Data
| ID | 15470690 |
|---|---|
| Authors | Ewa Sajnaga (0000-0003-4179-1948, John Paul II Catholic University of Lublin, corresponding author), Marcin Skowronek (0000-0003-2069-0347, John Paul II Catholic University of Lublin), Agnieszka Kalwasińska (0000-0002-2435-567X, Nicolaus Copernicus University), Waldemar Kazimierczak (0000-0001-8576-4701, John Paul II Catholic University of Lublin), Magdalena Lis (0000-0001-8566-2406, John Paul II Catholic University of Lublin), Monika Elżbieta Jach (0000-0002-4932-3260, John Paul II Catholic University of Lublin), Adrian Wiater (0000-0003-1433-3634, Maria Curie-Skłodowska University) |
| Year | 2022 |
| Volume | 19 |
| Issue | 6 |
| Pages | 3480-3480 |
| Publication date | 2022-03-15 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph19063480 |
| PMID | 35329164 |
| OpenAlex | W4220804178 |
| Language | EN |
| References cited | 63 |
Root-feeding Amphimallon solstitiale larvae and certain other scarab beetles are the main soil-dwelling pests found in Europe, while entomopathogenic nematodes (EPN) have been used as a biocontrol agent against these species. Our study provides the first detailed characterization of the bacterial community of the midgut in wild A. solstitiale larvae, based on the nanopore sequencing of the 16S rRNA gene. In the whole dataset, we detected 2586 different genera and 11,641 species, with only 83 diverse bacterial genera shared by all studied individuals, which may represent members of the core midgut microbiota of A. solstitiale larvae. Subsequently, we compared the midgut microbiota of EPN-resistant and T0 (prior to EPN exposure) individuals, hypothesizing that resistance to this parasitic infection may be linked to the altered gut community. Compared to the control, the resistant insect microbiota demonstrated lower Shannon and Evenness indices and significant differences in the community structure. Our studies confirmed that the gut microbiota alternation is associated with resistant insects; however, there are many processes involved that can affect the bacterial community. Further research on the role of gut microbiota in insect-parasitic nematode interaction may ultimately lead to the improvement of biological control strategies in insect pest management
16S ribosomal RNA · Bacteria · Biological pest control · Biology · Botany · Gut flora · Insect · Larva · Microbial population biology · Midgut · Zoology · Entomopathogenic Microorganisms in Pest Control · Insect Resistance and Genetics · Insect symbiosis and bacterial influences · Ecology · Genetics · Immunology · Microbiology
| Highly cited | No |
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