Methylation in the Promoter Region of the Dopamine Transporter DAT1 Gene in People Addicted to Nicotine

Bibliographic Data

ID15470830
AuthorsJolanta Chmielowiec (0000-0003-3285-5313, University of Zielona Góra), Krzysztof Chmielowiec (0000-0003-4254-5466, University of Zielona Góra), Aleksandra Strońska-Pluta (0000-0001-6564-2141, Pomeranian Medical University), Aleksandra Suchanecka (0000-0002-7137-1429, Pomeranian Medical University), Kinga Humińska‐Lisowska (0000-0003-0034-0809, Gdansk University of Physical Education and Sport), Milena Lachowicz (0000-0002-8402-0564, Gdansk University of Physical Education and Sport), Marta Niewczas (0000-0002-2687-3243, Rzeszów University), Monika Bialecka (0000-0002-3137-2224, Pomeranian Medical University), Małgorzata Śmiarowska (0000-0003-3180-5787, Pomeranian Medical University), Anna Grzywacz (0000-0002-2214-7199, Pomeranian Medical University, corresponding author)
Year2022
Volume19
Issue14
Pages8602-8602
Publication date2022-07-14
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph19148602
PMID35886451
OpenAlexW4286240433
LanguageEN
Citations received2
References cited65

The dopaminergic system is a crucial element of the addiction processes. The dopamine transporter modulates the dynamics and levels of released dopamine in the synaptic cleft. Therefore, regulation of dopamine transporter ( DAT1 ) gene expression is critical for maintaining homeostasis in the dopaminergic system. The aim of our study is evaluation of the methylation status of 33 CpG islands located in the DAT1 gene promoter region related to nicotine dependency. We investigated 142 nicotine-dependent subjects and 238 controls. Our results show that as many as 14 of the 33 CpG islands tested had statistically significantly higher methylation in the nicotine-dependent group compared to the control group. After applying Bonferroni correction, the total number of methylation sites was also significantly higher in the dependent subjects group. The analysis of the methylation status of particular CpG sites revealed a new direction of research regarding the biological aspects of nicotine addiction

Addiction · Biology · CpG site · DNA methylation · Dopamine · Dopamine transporter · Dopaminergic · Gene · Gene expression · Methylation · Nicotine · Cannabis and Cannabinoid Research · Epigenetics and DNA Methylation · Neuroscience · Psychology · Tryptophan and brain disorders · Endocrinology · Genetics

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