Quantification of Protozoa and Viruses from Small Water Volumes

Bibliographic Data

ID15480389
AuthorsJ Bonilla (Oceans and Human Health Center, University of Miami, Key Biscayne, FL 33149, USA), J Alfredo Bonilla (University of Wisconsin–River Falls, corresponding author), Tonya D Bonilla (University of Miami), Tonya Bonilla (Oceans and Human Health Center, University of Miami, Key Biscayne, FL 33149, USA), Amir M Abdelzaher (0000-0001-5888-4281, University of Miami), Amir Abdelzaher (Oceans and Human Health Center, University of Miami, Key Biscayne, FL 33149, USA), Troy M Scott (University of Miami), Troy Scott (Oceans and Human Health Center, University of Miami, Key Biscayne, FL 33149, USA), Jerzy Łukasik (Biological Consulting Services (United States)), Helena M Solo‐Gabriele (0000-0003-3390-3823, University of Miami), Carol Palmer (0000-0003-4612-5426, University of Miami)
Year2015
Volume12
Issue7
Pages7118-7132
Publication date2015-06-24
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph120707118
PMID26114244
PMCIDPMC4515645
OpenAlexW1530712798
LanguageEN
Citations received1
References cited47

Large sample volumes are traditionally required for the analysis of waterborne pathogens. The need for large volumes greatly limits the number of samples that can be processed. The aims of this study were to compare extraction and detection procedures for quantifying protozoan parasites and viruses from small volumes of marine water. The intent was to evaluate a logistically simpler method of sample collection and processing that would facilitate direct pathogen measures as part of routine monitoring programs. Samples were collected simultaneously using a bilayer device with protozoa capture by size (top filter) and viruses capture by charge (bottom filter). Protozoan detection technologies utilized for recovery of Cryptosporidium spp. and Giardia spp. were qPCR and the more traditional immunomagnetic separation-IFA-microscopy, while virus (poliovirus) detection was based upon qPCR versus plaque assay. Filters were eluted using reagents consistent with the downstream detection technologies. Results showed higher mean recoveries using traditional detection methods over qPCR for Cryptosporidium (91% vs. 45%) and poliovirus (67% vs. 55%) whereas for Giardia the qPCR-based methods were characterized by higher mean recoveries (41% vs. 28%). Overall mean recoveries are considered high for all detection technologies. Results suggest that simultaneous filtration may be suitable for isolating different classes of pathogens from small marine water volumes. More research is needed to evaluate the suitability of this method for detecting pathogens at low ambient concentration levels

Biology · Chromatography · Cryptosporidium · Feces · Filtration (mathematics · Giardia · Immunomagnetic separation · Norovirus · Poliovirus · Protozoa · Virus · Chemistry · Fecal contamination and water quality · Parasitic Infections and Diagnostics · Viral gastroenteritis research and epidemiology · Microbiology · Virology

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