Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats
Bibliographic Data
| ID | 15480925 |
|---|---|
| Authors | Ren Lin (0000-0002-9402-0883, China Medical University), Yue Jia (0000-0001-8013-4540, China Medical University), Fengjuan Wu (China Medical University), Yuan Meng (0000-0002-0963-0581, China Medical University), Meng Yuan (0000-0001-9706-3814, China Medical University), Qi Sun (0000-0003-2222-6505, China Medical University), Lihong Jia (0000-0001-5675-0650, China Medical University, corresponding author) |
| Year | 2019 |
| Volume | 16 |
| Issue | 21 |
| Pages | 4152-4152 |
| Publication date | 2019-10-28 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph16214152 |
| PMID | 31661889 |
| OpenAlex | W2981850988 |
| Language | EN |
| References cited | 48 |
The aim of this study was to investigate whether combined exposure to fructose and bisphenol A (BPA) has a synergistic effect on abnormal lipid metabolism in the liver of developmental male rats and its possible mechanism. Fifty weaned male Wistar rats were divided into five groups: the control, 13% fructose, 20% fructose, 1 μg/mL BPA, and 13% fructose + 1 μg/mL BPA (combined exposure). Rats were exposed to fructose and/or BPA through drinking water for eight weeks. Genes or proteins regulating lipid metabolism include sterol regulatory element binding protein 1 (SREBP1), adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), zinc α 2 glycoprotein (ZAG) and estrogen receptor α (ERα), and the expression of proteins regulating inflammatory response, such as TLR4 and NF-κB, were determined. Serum total cholesterol (T-CHO), triglyceride (TG), low, high density lipoprotein cholesterol (LDL-C, HDL-C), blood glucose, insulin, IL-17 and TNF-α levels were also measured. Liver tissue morphology was observed by H&E staining. The results showed that the levels of gene and protein catalyzing lipogenesis were increased (SREBP1, ACC1 and FAS), while those catalyzing lipolysis were decreased (ATGL, HSL and ZAG), accompanied by dyslipidemia, insulin resistance and hepatic fat accumulation, and there were higher expression of TLR4 and NF-κB protein and lower expression of ERα protein in liver, and increased serum IL-17 and TNF-α levels in fructose and/or BPA exposed rats compared with controls. Moreover, the above indicators were more serious in combined exposure group than in single exposure group. Therefore, abnormal lipid metabolism in the liver of developmental rats could be exacerbated by combined exposed to fructose and BPA
Adipose tissue · Adipose triglyceride lipase · Biology · Carbohydrate Metabolism · Carbohydrate-responsive element-binding protein · Cholesterol · Fatty acid synthase · Fructose · Hormone-sensitive lipase · Lipid metabolism · Lipogenesis · Lipolysis · Lipoprotein lipase · Sterol · Sterol regulatory element-binding protein · Triglyceride · Chemistry · Diet and metabolism studies · Diet, Metabolism, and Disease · Effects and risks of endocrine disrupting chemicals · Medicine · Biochemistry · Endocrinology · Internal Medicine
| Citation velocity | historical |
|---|---|
| Highly cited | No |