Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dams in the Eastern Cape Province of South Africa
Bibliographic Data
| ID | 15493580 |
|---|---|
| Authors | Vincent N Chigor (0000-0002-0811-4526, University of Fort Hare), Vincent Chigor (University of Fort Hare), Anthony I Okoh (0000-0002-9770-085X, University of Fort Hare, corresponding author) |
| Year | 2012 |
| Volume | 9 |
| Issue | 11 |
| Pages | 4017-4032 |
| Publication date | 2012-11-05 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph9114017 |
| PMID | 23202829 |
| PMCID | PMC3524610 |
| OpenAlex | W2057431394 |
| Language | EN |
| Citations received | 4 |
| References cited | 63 |
Human enteric viruses (HEntVs) are a major cause of water-related diseases. The prevalence of hepatitis A virus (HAV), rotaviruses (RoV) and enteroviruses (EnV) in Buffalo River waters was assessed quantitatively over a period of 12 months (August 2010 to July 2011). Seventy-two samples were collected from six sites, including three dams, and concentrated using the adsorption-elution method. Viral RNA was extracted using a commercial kit, and the viruses were quantified by real-time quantitative reverse transcriptase PCR (RT-qPCR). Two or more viruses were detected in 12.5% of the samples. HAV was detected in 43.1% of the samples and in significantly (p < 0.05) varying concentrations of 1.5 × 10(1)–1.9 × 10(5) genome copies/L compared to RoV and EnV, while RoVs were detected in 13.9% of samples, with concentrations ranging from 2.5 × 10(1)–2.1 × 10(3) genome copies/L, and EnV were detected in 9.7% of the samples, with concentrations ranging from 1.3 × 10(1)–8.6 × 10(1) genome copies/L. Only HAV was detected at all the sites, with the Bridle Drift Dam recording significantly higher (p < 0.05) concentrations. The presence of enteric viruses in Buffalo River may constitute public health risks and the incidence of HAV at all the sites could reflect both the epidemiological status of hepatitis A and HAV persistence in the water environments
Biology · Feces · Hepatitis a virus · Veterinary medicine · Virus · Hepatitis Viruses Studies and Epidemiology · Medicine · Viral gastroenteritis research and epidemiology · Viral Infections and Immunology Research · Microbiology · Virology
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| Citations per year | 0.36 |
|---|---|
| Citation span | 2015 - 2020 (6) |
| Highly cited | No |