Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae
Purification, Characterization and Antileukemic Activity
Bibliographic Data
| ID | 15503152 |
|---|---|
| Authors | Hani Moubasher (0000-0002-1319-4698, Cairo University), Hani A Moubasher (Cairo University), Bassem A Balbool (0000-0002-9699-3558, October University of Modern Sciences and Arts, corresponding author), Yosra A Helmy (0000-0003-1470-5418, Suez Canal University), Amnah Mohammed Alsuhaibani (0000-0002-8299-1906, Princess Nourah bint Abdulrahman University), A A Atta (0000-0003-4939-404X, Taif University), Donia H Sheir (0000-0002-9241-8484, Chemistry of Natural and Microbial Products Department, National Research Center, Giza 12622, Egypt), Ahmed M Abdel-Azeem (0000-0003-2897-3966, Suez Canal University, corresponding author) |
| Year | 2022 |
| Volume | 19 |
| Issue | 2 |
| Pages | 680-680 |
| Publication date | 2022-01-07 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph19020680 |
| PMID | 35055502 |
| OpenAlex | W4206257549 |
| Language | EN |
| References cited | 53 |
Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae . The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a K m of 9.37 μM and V max of 127.00 μM/mL/min. The purified asparaginase showed an IC 50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC 50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, cost-effective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents
Asparaginase · Biology · Botany · Cell culture · Chromatography · Enzyme · Enzyme assay · IC50 · In vitro · Lasiodiplodia theobromae · Leukemia · Lymphoblastic Leukemia · Plant use of endophytic fungi in defense · Acute Lymphoblastic Leukemia research · Cancer Research and Treatments · Chemistry · Transgenic Plants and Applications · Biochemistry · Immunology · Microbiology
| Highly cited | No |
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