Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation

Evaluation of Greenness

Bibliographic Data

ID15510544
AuthorsMahesh Attimarad (0000-0002-7007-9119, King Faisal University, corresponding author), Anroop B Nair (0000-0003-2850-8669, King Faisal University), Nagaraja Sreeharsha (0000-0002-2058-255X, King Faisal University), Bandar E Al‐Dhubiab (0000-0002-2684-4186, King Faisal University), Katharigatta N Venugopala (0000-0003-0680-1549, Durban University of Technology), Pottathil Shinu (0000-0003-4245-0803, King Faisal University)
Year2021
Volume18
Issue2
Pages448-448
Publication date2021-01-08
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph18020448
PMID33429964
OpenAlexW3118643531
LanguageEN
References cited4

The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were based on the peak amplitude difference and first-order derivative absorption of the ratio spectra developed by the manipulation of scanned UV spectra. REM and MET showed good linearity in the series of 1-20 μg ml -1 and 2.5-35 μg ml -1 , respectively, by all three methods with an excellent correlation coefficient (r 2 ≥ 0.998). Further, the proposed UV spectroscopic techniques were validated as per International Council for Harmonization guidelines. The methods showed good sensitivity, accuracy, and precision. Anticipated procedures were effectively utilized for the concurrent quantification of REM and MET in laboratory prepared mixtures and tablets. The high percent recovery with low standard deviation found for both analytes by all three methods confirms the accuracy and precision of the procedures. Finally, the greenness of the proposed spectroscopic methods, evaluated by semi-quantitative and quantitative methods, showed the eco-friendly nature of the methods. Furthermore, the proposed approaches were simple, accurate, sensitive, economic, and environmentally friendly and hence can be utilized for regular quality control of REM and MET formulation

Accuracy and precision · Analyte · Analytical Chemistry (journal · Chromatography · Correlation coefficient · Derivative (finance · Environmentally Friendly · Linearity · Physics · Second derivative · Solvent · Statistics · Analytical Chemistry and Chromatography · Analytical Methods in Pharmaceuticals · Chemistry · Electrochemical sensors and biosensors · Mathematics · Organic Chemistry

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