A Comparison of the Human Buccal Cell Assay and the Pollen Abortion Assay in Assessing Genotoxicity in an Urban-Rural Gradient

Bibliographic Data

ID15511840
AuthorsAlan da Silveira Fleck (0000-0001-9290-264X, Universidade Federal de Ciências da Saúde de Porto Alegre), Alan Fleck (Oxidative Stress and Air Pollution Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Sarmento Leite Street, 245, Room 9. Porto Alegre 90050-170, Rio Grande do Sul, Brazil), Mariana Vieira (0000-0002-2406-3159, Universidade Federal de Ciências da Saúde de Porto Alegre), Sérgio L Amantéa (0000-0002-1256-3957, Universidade Federal de Ciências da Saúde de Porto Alegre), Sergio Amantéa (Oxidative Stress and Air Pollution Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Sarmento Leite Street, 245, Room 9. Porto Alegre 90050-170, Rio Grande do Sul, Brazil), Cláudia Ramos Rhoden (0000-0002-3099-9375, Universidade Federal de Ciências da Saúde de Porto Alegre, corresponding author), Claudia Rhoden (Oxidative Stress and Air Pollution Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Sarmento Leite Street, 245, Room 9. Porto Alegre 90050-170, Rio Grande do Sul, Brazil)
Year2014
Volume11
Issue9
Pages8825-8838
Publication date2014-08-27
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph110908825
PMID25166920
PMCIDPMC4198993
OpenAlexW2042242810
LanguageEN
References cited33

Air pollution is exacerbated near heavy traffic roads in cities. Air pollution concentration and composition vary by region and depend on urban-rural gradients. The aim of this study was to evaluate the distribution of air pollution in areas of varying population densities and to compare plant biomonitoring with an established biomarker of human exposure to traffic-related air pollution in children. The areas of study were selected near a major street in 3 different regions. Areas A, B and C represent high, intermediate and low population densities, respectively. Micronucleus assay, an established biomarker of human exposure, was performed in children from these areas. For a plant biomonitoring assay, the pollen abortion assay was performed on Bauhinia variegata in these areas. NO2 and O3 concentrations were determined by passive sampling. We report here that the pollen abortion frequency in Bauhinia variegata is correlated with NO2 concentration (P = 0.004) and is strongly associated with vehicular flow and population density in the studied areas. Micronuclei frequency in buccal cells of children was higher in the regions with more degree of urbanization (P < 0.001) following the same pattern of O3 concentrations (P = 0.030). In conclusion, our results demonstrate that high concentrations of air pollutants in Porto Alegre are related to both human and plant genotoxicity. Areas with different concentration of pollutants demonstrated to have an urbanization gradient dependent pattern which also reflected on genotoxic damage among these areas

Air pollution · Biology · Biomonitoring · Botany · Buccal swab · Environmental health · Genotoxicity · Micronucleus test · Pollen · Pollutant · Population · Toxicity · Urbanization · Air Quality and Health Impacts · Carcinogens and Genotoxicity Assessment · Chemistry · Environmental Science · Medicine · Noise Effects and Management · Ecology · Environmental Chemistry · Molecular Biology · Pollution · Toxicology

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