Effect of Chlorine Exposure on the Survival and Antibiotic Gene Expression of Multidrug Resistant Acinetobacter baumannii in Water
Bibliographic Data
| ID | 15512558 |
|---|---|
| Authors | Deepti Prasad Karumathil (University of Connecticut), Deepti Karumathil (University of Connecticut), Hsin‐Bai Yin (0000-0002-1175-4516, University of Connecticut), Hsin-Bai Yin (University of Connecticut), Anup Kollanoor Johny (0000-0002-4713-5506, University of Minnesota), Kumar Venkitanarayanan (0000-0001-5966-7852, University of Connecticut, corresponding author) |
| Year | 2014 |
| Volume | 11 |
| Issue | 2 |
| Pages | 1844-1854 |
| Publication date | 2014-02-07 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph110201844 |
| PMID | 24514427 |
| PMCID | PMC3945572 |
| OpenAlex | W2059225967 |
| Language | EN |
| Citations received | 2 |
| References cited | 36 |
Acinetobacter baumannii is a multidrug resistant pathogen capable of causing a wide spectrum of clinical conditions in humans. Acinetobacter spp. is ubiquitously found in different water sources. Chlorine being the most commonly used disinfectant in water, the study investigated the effect of chlorine on the survival of A. baumannii in water and transcription of genes conferring antibiotic resistance. Eight clinical isolates of A. baumannii, including a fatal meningitis isolate (ATCC 17978) (~108 CFU/mL) were separately exposed to free chlorine concentrations (0.2, 1, 2, 3 and 4 ppm) with a contact time of 30, 60, 90 and 120 second. The surviving pathogen counts at each specified contact time were determined using broth dilution assay. In addition, real-time quantitative PCR (RT-qPCR) analysis of the antibiotic resistance genes (efflux pump genes and those encoding resistance to specific antibiotics) of three selected A. baumannii strains following exposure to chlorine was performed. Results revealed that all eight A. baumannii isolates survived the tested chlorine levels during all exposure times (p > 0.05). Additionally, there was an up-regulation of all or some of the antibiotic resistance genes in A. baumannii, indicating a chlorine-associated induction of antibiotic resistance in the pathogen
Acinetobacter · Acinetobacter baumannii · Antibiotics · Bacteria · Biology · Gene · Multiple drug resistance · Pseudomonas aeruginosa · Antibiotic Resistance in Bacteria · Bacterial biofilms and quorum sensing · Pharmaceutical and Antibiotic Environmental Impacts · Genetics · Microbiology
| Unique citing works | 2 |
|---|---|
| Citations per year | 0,17 |
| Citation span | 2014 - 2023 (10) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 2 |