Sars-CoV-2 RNA Detection on Environmental Surfaces in a University Setting of Central Italy
Bibliographic Data
| ID | 15513067 |
|---|---|
| Authors | Anna Casabianca (0000-0001-6063-7466, University of Urbino, corresponding author), Chiara Orlandi (0000-0003-3288-7286, University of Urbino), Giulia Amagliani (0000-0002-0547-5107, University of Urbino), Mauro Magnani (0000-0001-6456-6626, University of Urbino), Giorgio Brandi (0000-0003-1380-6137, University of Urbino), Giuditta Fiorella Schiavano (0000-0002-1427-3486, University of Urbino) |
| Year | 2022 |
| Volume | 19 |
| Issue | 9 |
| Pages | 5560-5560 |
| Publication date | 2022-05-03 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph19095560 |
| PMID | 35564956 |
| OpenAlex | W4225320231 |
| Language | EN |
| References cited | 28 |
The transmission of SARS-CoV-2 occurs through direct contact (person to person) and indirect contact by means of objects and surfaces contaminated by secretions from individuals with COVID-19 or asymptomatic carriers. In this study, we evaluated the presence of SARS-CoV-2 RNA on surfaces made of different materials located in university environments frequented by students and staff involved in academy activity during the fourth pandemic wave (December 2021). A total of 189 environmental samples were collected from classrooms, the library, computer room, gym and common areas and subjected to real-time PCR assay to evaluate the presence of SARS-CoV-2 RNA by amplification of the RNA-dependent RNA polymerase (RdRp) gene. All samples gave a valid result for Internal Process Control and nine (4.8%) tested very low positive for SARS-CoV-2 RNA amplification with a median Ct value of 39.44 [IQR: 37.31-42.66] (≤1 copy of viral genome). Our results show that, despite the prevention measures implemented, the presence of infected subjects cannot be excluded, as evidenced by the recovery of SARS-CoV-2 RNA from surfaces. The monitoring of environmental SARS-CoV-2 RNA could support public health prevention strategies in the academic and school world
Asymptomatic · Biology · Coronavirus disease 2019 (COVID-19 · Disease · Gene · Pandemic · RNA · RNA-dependent RNA polymerase · Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 · Transmission (telecommunications · Computer Science · COVID-19 epidemiological studies · Infection Control and Ventilation · Medicine · SARS-CoV-2 detection and testing · Genetics · Internal Medicine · Virology
Effects of Covid-19 on business and research
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Stability of Sars-CoV-2 in different environmental conditions
Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents
Effects of Covid-19 pandemic in daily life
Air, Surface Environmental, and Personal Protective Equipment Contamination by Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-CoV-2) From a Symptomatic Patient
Aerosol and Surface Stability of Sars-CoV-2 as Compared with Sars-CoV-1
First Detection of Severe Acute Respiratory Syndrome Coronavirus 2 on the Surfaces of Tourist-Recreational Facilities in Italy
| Highly cited | No |
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