Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia
Bibliographic Data
| ID | 15514004 |
|---|---|
| Authors | Fatin Aina Zulkhairi Amin (0000-0002-0671-0316, Universiti Putra Malaysia), Suriana Sabri (0000-0003-4027-5730, Universiti Putra Malaysia), Maznah Ismail (0000-0002-2378-0519, Universiti Putra Malaysia), Kim Wei Chan (0000-0003-3028-0800, Universiti Putra Malaysia), Norsharina Ismail (0000-0002-2580-8300, Universiti Putra Malaysia), Mohd Esa Norhaizan (0000-0003-1545-0306, Universiti Putra Malaysia), Mohd Azmi Mohd Lila (0000-0001-7153-1026, Universiti Putra Malaysia), Norhasnida Zawawi (0000-0001-7459-5025, Universiti Putra Malaysia, corresponding author) |
| Year | 2019 |
| Volume | 17 |
| Issue | 1 |
| Pages | 278-278 |
| Publication date | 2019-12-31 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph17010278 |
| PMID | 31906055 |
| OpenAlex | W2998108123 |
| Language | EN |
| Citations received | 2 |
| References cited | 3 |
This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama . Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health
Antimicrobial · Apidae · Bacillus (shape) · Bacillus amyloliquefaciens · Bacillus subtilis · Bacteria · Biology · Botany · Fermentation · Gram · Lactobacillus · Lactobacillus rhamnosus · Probiotic · Stingless bee · Bee Products Chemical Analysis · Insect and Pesticide Research · Probiotics and Fermented Foods · Food Science · Microbiology
| Citations per year | 0.25 |
|---|---|
| Citation span | 2018 - 2023 (6) |
| Highly cited | No |