The Role of miRNAs in Zearalenone-Promotion of TM3 Cell Proliferation

Bibliographic Data

ID15514717
AuthorsWanglong Zheng (0000-0002-0939-331X, Yangzhou University), Wentong Fan (Yangzhou University), Nannan Feng (0000-0002-0212-6171, Yangzhou University), Nanyan Lu (0000-0002-1128-2934, Kansas State University), Hui Zou (0000-0002-0212-3894, Yangzhou University), Jianhong Gu (0000-0002-3226-4604, Yangzhou University), Yan Yuan (0000-0003-4323-6841, Yangzhou University), Xuezhong Liu (Yangzhou University), Jianfa Bai (0000-0002-0021-3036, Kansas State University), Jianchun Bian (0000-0001-8023-0751, Yangzhou University, corresponding author), Zongping Liu (0000-0001-9071-8363, Yangzhou University, corresponding author)
Year2019
Volume16
Issue9
Pages1517-1517
Publication date2019-04-29
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph16091517
PMID31035709
OpenAlexW2940607677
LanguageEN
References cited54

Zearalenone (ZEA) is a non-steroidal estrogen mycotoxin produced by several Gibberella and Fusarium species. Accumulating evidence has indicated that ZEA strongly stimulates cell proliferation. However the detailed molecular and cellular mechanisms of ZEA-mediated induction of cell proliferation have not yet been completely explained. The aim of this study was to detect the role of miRNAs in ZEA-mediated induction of cell proliferation. The effects of ZEA on cell proliferation were assessed using a cell counting kit assay and xCELLigence system. Micro-RNA sequencing was performed after treatment of TM3 cells with ZEA (0.01 μmol/L) for different time periods (0, 2, 6 and 18 h). Cell function and pathway analysis of the miRNA target genes were performed by Ingenuity Pathway Analysis (IPA). We found that ZEA promotes TM3 cell proliferation at low concentrations. miRNA sequenceing revealed 66 differentially expressed miRNAs in ZEA-treated cells in comparison to the untreated control ( p p < 0.05) in ZEA-treated groups. IPA analysis showed that the predicated miRNAs target gene involved in cell Bio-functions including cell cycle, growth and proliferation, and in signaling pathways including MAPK and RAS-RAF-MEK-ERK pathways. Results from flow cytometry and Western Blot analysis validated the predictions that ZEA can affect cell cycle, and the MAPK signaling pathway. Taking these together, the cell proliferation induced ZEA is regulated by miRNAs. The results shed light on the molecular and cellular mechanisms for the mediation of ZEA to induce proliferation

Biology · Cell growth · Computational biology · Gene · microRNA · Mycotoxin · Political science · Promotion (chess · Zearalenone · Chemistry · Garlic and Onion Studies · Mycotoxins in Agriculture and Food · Plant Disease Resistance and Genetics · Biotechnology · Cancer Research · Cell Biology · Genetics

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