Immobilization of EreB on Acid-Modified Palygorskite for Highly Efficient Degradation of Erythromycin
Bibliographic Data
| ID | 15514732 |
|---|---|
| Authors | Shensheng Ni (Changzhou University), Chunyu Li (0000-0002-3515-446X, Changzhou University), Yicheng Yu (Jiangsu Normal University), Dongze Niu (0000-0001-6554-885X, Changzhou University), Jie Zhu (0000-0002-5477-172X, Changzhou University), Dongmin Yin (0000-0001-9329-050X, Changzhou University), Chongqing Wang (0000-0003-2580-9263, National Animal Husbandry Service), Wenfan Zhang (0000-0002-6084-3574, Changzhou University), Xingmei Jiang (Qingdao Institute of Animal Husbandry and Veterinary Medicine), Jianjun Ren (0000-0002-5938-688X, Changzhou University, corresponding author) |
| Year | 2022 |
| Volume | 19 |
| Issue | 17 |
| Pages | 11064-11064 |
| Publication date | 2022-09-04 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph191711064 |
| PMID | 36078780 |
| OpenAlex | W4294739201 |
| Language | EN |
| References cited | 45 |
Erythromycin is one of the most commonly used macrolide antibiotics. However, its pollution of the ecosystem is a significant risk to human health worldwide. Currently, there are no effective and environmentally friendly methods to resolve this issue. Although erythromycin esterase B (EreB) specifically degrades erythromycin, its non-recyclability and fragility limit the large-scale application of this enzyme. In this work, palygorskite was selected as a carrier for enzyme immobilization. The enzyme was attached to palygorskite via a crosslinking reaction to construct an effective erythromycin-degradation material (i.e., EreB@modified palygorskite), which was characterized using FT-IR, SEM, XRD, and Brunauer-Emmett-Teller techniques. The results suggested the successful modification of the material and the loading of the enzyme. The immobilized enzyme had a higher stability over varying temperatures (25-65 °C) and pH values (6.5-10.0) than the free enzyme, and the maximum rate of reaction (V max ) and the turnover number (k cat ) of the enzyme increased to 0.01 mM min -1 and 169 min -1 , respectively, according to the enzyme-kinetics measurements. The EreB@modified palygorskite maintained about 45% of its activity after 10 cycles, and degraded erythromycin in polluted water to 20 mg L -1 within 300 min. These results indicate that EreB could serve as an effective immobilizing carrier for erythromycin degradation at the industrial scale
Active site · Adsorption · Antibiotics · Chromatography · Degradation (telecommunications · Enzyme · Enzyme Kinetics · Erythromycin · Nuclear chemistry · Palygorskite · Analytical chemistry methods development · Chemistry · Electrochemical sensors and biosensors · Enzyme Catalysis and Immobilization · Biochemistry · Environmental Chemistry · Organic Chemistry
| Citation velocity | historical |
|---|---|
| Highly cited | No |