Sampling Considerations for Wastewater Surveillance of Antibiotic Resistance in Fecal Bacteria
Bibliographic Data
| ID | 15515520 |
|---|---|
| Authors | P M C Huijbers (University of Gothenburg), Julián Bobis Camacho (0009-0002-5030-2018, University of Gothenburg), Marion Hutinel (0000-0003-4548-7724, University of Gothenburg), D G Joakim Larsson (0000-0002-5496-0328, University of Gothenburg), Carl‐Fredrik Flach (0000-0002-4101-5095, University of Gothenburg, corresponding author) |
| Year | 2023 |
| Volume | 20 |
| Issue | 5 |
| Pages | 4555-4555 |
| Publication date | 2023-03-04 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | International Journal of Environmental Research and Public Health (JOURNAL) |
| Journal identifiers | ISSN: 1661-7827 • E-ISSN: 1660-4601 |
| Publisher | Multidisciplinary Digital Publishing Institute (PUBLISHER • CH) |
| DOI | 10.3390/ijerph20054555 |
| PMID | 36901565 |
| OpenAlex | W4323317748 |
| Language | EN |
| Citations received | 2 |
| References cited | 1 |
Wastewaters can be analyzed to generate population-level data for public health surveillance, such as antibiotic resistance monitoring. To provide representative data for the contributing population, bacterial isolates collected from wastewater should originate from different individuals and not be distorted by a selection pressure in the wastewater. Here we use Escherichia coli diversity as a proxy for representativeness when comparing grab and composite sampling at a major municipal wastewater treatment plant influent and an untreated hospital effluent in Gothenburg, Sweden. All municipal samples showed high E. coli diversity irrespective of the sampling method. In contrast, a marked increase in diversity was seen for composite compared to grab samples from the hospital effluent. Virtual resampling also showed the value of collecting fewer isolates on multiple occasions rather than many isolates from a single sample. Time-kill tests where individual E. coli strains were exposed to sterile-filtered hospital wastewater showed rapid killing of antibiotic-susceptible strains and significant selection of multi-resistant strains when incubated at 20 °C, an effect which could be avoided at 4 °C. In conclusion, depending on the wastewater collection site, both sampling method and collection/storage temperature could significantly impact the representativeness of the wastewater sample
Antibiotic resistance · Antibiotics · Biology · Effluent · Environmental health · Population · Representativeness heuristic · Sewage treatment · Statistics · Veterinary medicine · Wastewater · Environmental Science · Fecal contamination and water quality · Gut microbiota and health · Mathematics · Medicine · Pharmaceutical and Antibiotic Environmental Impacts · Environmental Engineering · Microbiology
| Citations per year | 1 |
|---|---|
| Citation span | 2024 - 2025 (2) |
| Highly cited | No |