Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

Bibliographic Data

ID15515992
AuthorsLing Guo (0000-0003-1155-6170, Ministry of Education of the People's Republic of China), Jiajin Lin (0000-0002-1723-2460, Ministry of Education of the People's Republic of China), Jia-Jin Lin (Air Force Medical University), Yizhe Xue (Air Force Medical University), Yi-Zhe Xue (Air Force Medical University), Guangzhou An (Ministry of Education of the People's Republic of China), Guang-Zhou An (Air Force Medical University), Junping Zhang (0000-0002-7271-8615, Air Force Medical University), Jun-ping Zhang (Air Force Medical University), Keying Zhang (0000-0002-2909-3357, Air Force Medical University), Ke-Ying Zhang (Air Force Medical University), Wei He (0000-0001-8290-7981, Ministry of Education of the People's Republic of China), Huan Wang (0000-0003-3219-7226, Ministry of Education of the People's Republic of China), Wei Li (0000-0002-3581-849X, Air Force Medical University, corresponding author), Guirong Ding (0000-0001-5750-6000, Ministry of Education of the People's Republic of China, corresponding author)
Year2019
Volume16
Issue7
Pages1286-1286
Publication date2019-04-10
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueInternational Journal of Environmental Research and Public Health (JOURNAL)
Journal identifiersISSN: 1661-7827 • E-ISSN: 1660-4601
PublisherMultidisciplinary Digital Publishing Institute (PUBLISHER • CH)
DOI10.3390/ijerph16071286
PMID30974849
OpenAlexW2938794089
LanguageEN
References cited23

Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m2 on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin-eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect

Andrology · Biology · Field (mathematics · Physics · Pulsed radiofrequency · Quality (philosophy · Sperm · Sperm quality · Electromagnetic Fields and Biological Effects · Mathematics · Medicine · Reproductive Biology and Fertility · Sperm and Testicular Function · Surgery

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