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Frecuencia de genes que codifican proteínas autotransportadoras serinproteasa de Enterobacteriaceae (SPATE) en cepas de Escherichia coli difusamente adherente (Daec) provenientes de niños con y sin diarrea

Bibliographic Data

ID15534202
AuthorsKaren Ocampo (0000-0001-7543-9307, Federico Villarreal National University, corresponding author), Maribel Riveros (0000-0002-5347-3261, Federico Villarreal National University), María Pinedo-Bardales (0000-0002-0169-5620, Universidad Peruana Cayetano Heredia), Joaquı́m Ruiz (0000-0002-4431-2036, Universidad Científica del Sur), Theresa J Ochoa (0000-0002-3227-3906, Universidad Peruana Cayetano Heredia)
Year2021
Volume38
Issue1
Pages124-9
Publication date2021-03-26
Peer ReviewedYes
Open AccessYes
TypeARTICLE
VenueRevista Peruana de Medicina Experimental y Salud Pública (JOURNAL)
Journal identifiersISSN: 1726-4634 • E-ISSN: 1726-4642
PublisherNational Institute of Health of Peru (PUBLISHER • PE)
DOI10.17843/rpmesp.2021.381.5750
PMID34190904
OpenAlexW3136747705
LanguageES
References cited2

The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a control group. For the detection of the genes, 2 multiple PCRs were standardized: triple A (sigA, pet, espP) and triple B (sat, pic, espC). In both groups, the most frequent SPATE gene was Sat (39.7% of WD and 41.5% of WOD), followed by spP (20.6% and 9.7% in WD and WOD respectively). The other genes were detected in proportions lower than 10.0%, in the following order of frequency: pet, sigA, espC and pic, without significant differences between the groups. It was concluded that Sat is the most frequent SPATE in DAEC and that these strains may possess SPATE genes regardless of whether they are isolated in WD or WOD

Biology · Enterobacteriaceae · Escherichia coli · Gene · Humanities · Enterobacteriaceae and Cronobacter Research · Escherichia coli research studies · Philosophy · Viral gastroenteritis research and epidemiology · Genetics · Molecular Biology

  • Patrones de adherencia de cepas de Escherichia coli Difusamente adherente (Daec) provenientes de niños con y sin diarrea

    Open Access•Maribel Riveros, Francesca Barletta et al.•Revista Peruana de Medicina…•2011

Citation velocityhistorical
Highly citedNo

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