Evaluación de una prueba de Elisa con antígeno metabólico de Fasciola hepatica para el diagnóstico de fasciolosis humana en Cajamarca, Perú
Bibliographic Data
| ID | 15534392 |
|---|---|
| Authors | Hernán Cornejo (Dirección Regional de Salud del Callao, corresponding author), Fátima Oblitas, Sandro Cruzado, William Quispe (0009-0006-0492-8975, Instituto Nacional de Salud) |
| Year | 2010 |
| Volume | 27 |
| Issue | 4 |
| Pages | 569-574 |
| Publication date | 2010-12-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Revista Peruana de Medicina Experimental y Salud Pública (JOURNAL) |
| Journal identifiers | ISSN: 1726-4634 • E-ISSN: 1726-4642 |
| Publisher | National Institute of Health of Peru (PUBLISHER • PE) |
| DOI | 10.1590/s1726-46342010000400012 |
| PMID | 21308197 |
| OpenAlex | W2171108914 |
| SciELO | S1726-46342010000400012 |
| Language | ES |
| Citations received | 1 |
| References cited | 3 |
Metabolic (excretion/secretion) antigen was obtained from sheep infected with Fasciola hepatica, with a 1005 μg/μL of protein concentration, composed principally by proteins of molecular weight between 1.2 and 170 KDa. Bands of 170, 150, 31, 24, 18-14 and 10 kDa were detected. With this antigen an ELISA test was developed and the cut off was determined in 0.140. We evaluated 33 serums of patient with fascioliasis confirmed by visualization of eggs in feces, 177 serums of persons without fascioliasis from endemic rural areas of Cajamarca and 88 serums of patients with others parasitic and bacterial infections. We found a 97.0% of sensitivity, 96.6 specificity, 78.1% predictive positive value, 99.6 % predictive negative value. In 9/88 serums was found cross reactions. We recommended the implementation and use of this test for the fascioliasis diagnosis
Antigen · Biology · Fasciola · Fasciola hepatica · Fasciolosis · Feces · Helminths · Hepatica · Predictive value · Coccidia and coccidiosis research · Helminth infection and control · Medicine · Immunology · Internal Medicine · Microbiology
| Citations per year | 0.07 |
|---|---|
| Citation span | 2011 - 2011 (1) |
| Highly cited | No |