Comparación de dos protocolos de extracción de ADN de Trypanosoma cruzi cultivados en medio axénico
Bibliographic Data
| ID | 15534666 |
|---|---|
| Authors | Mariela López (University of Carabobo, corresponding author), María Gabriela Rivera (University of Carabobo), Mercedes Viettri (University of Carabobo), María Lares (University of Carabobo), Antonio Morocoima (0000-0002-9341-547X, Universidad de Oriente), Leidi HERRERA (0000-0001-8515-2078, Central University of Venezuela), Elizabeth Ferrer (0000-0002-4173-6642, University of Carabobo) |
| Year | 2014 |
| Volume | 31 |
| Issue | 2 |
| Pages | 222-7 |
| Publication date | 2014-07-02 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Revista Peruana de Medicina Experimental y Salud Pública (JOURNAL) |
| Journal identifiers | ISSN: 1726-4634 • E-ISSN: 1726-4642 |
| Publisher | National Institute of Health of Peru (PUBLISHER • PE) |
| DOI | 10.17843/rpmesp.2014.312.38 |
| PMID | 25123858 |
| OpenAlex | W1979095563 |
| Language | ES |
| References cited | 1 |
Although the technique of Chelex100 provided less purity and integrity of DNA, it allowed a successful amplification of kDNA by PCR, avoiding the use of laborious techniques and toxic organic solvents
Agarose gel electrophoresis · Biology · Chromatography · DNA · DNA extraction · Extraction (chemistry · Kinetoplast · Polymerase chain reaction · Trypanosoma cruzi · Chemistry · Helminth infection and control · Parasitic Infections and Diagnostics · Trypanosoma species research and implications · Biochemistry · Molecular Biology
| Highly cited | No |
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