Accuracy of Capillary Blood for Assessing Vitamin A Nutritional Status Among Children Under 7 Years of Age

A Multicenter Study

Datos Bibliográficos

ID15535720
AutoresLuanluan Li (0009-0008-1371-6370, Shanghai Jiao Tong University), Song Hu (0000-0002-6750-6992, Shanghai Jiao Tong University), Xiaonan Li (0000-0002-3033-3119, Second Affiliated Hospital of Nanjing Medical University), Feiyong Jia (0000-0002-8309-1395, Jilin University), Chi Mei-zhu (Wenzhou Medical University), Zhihong Wen (0000-0003-4762-485X, The People's Hospital of Guangxi Zhuang Autonomous Region), Sufei Yang (0000-0002-5534-5554, Sichuan University), Yuning Li (0000-0003-3679-8133, First Hospital of Lanzhou University), L J Ha (Yinchuan First People's Hospital), Ying Yang (0000-0002-3751-2590, Dalian Maternal and Child Health Hospital), Xiaoling Long (Shenzhen Maternity and Child Healthcare Hospital), Shuanfeng Fang (0000-0002-1511-652X, Zhengzhou University), Lu Xie (0000-0001-7541-2243, Yunnan Maternal and Child Health), Huifeng Zhang (0000-0002-9818-7904, Hebei Medical University), Xiaodan Yu (0000-0002-1629-976X, Shanghai Jiao Tong University, autor de correspondencia)
Año2025
Volumen21
Número2
Páginase13796-e13796
Fecha de publicación2025-01-03
Peer ReviewedSí
Open AccessSí
TipoARTICLE
RevistaMaternal and Child Nutrition (JOURNAL)
Identificadores de la revistaISSN: 1740-8695 • E-ISSN: 1740-8709
EditorialWiley (PUBLISHER • GB)
DOI10.1111/mcn.13796
PMID39749822
OpenAlexW4406021246
IdiomaEN
Referencias citadas2

Vitamin A deficiency remains a major public health problem worldwide, particularly among young children. Capillary blood has the potential for application in vitamin A assessment. The aim of this study is to validate the accuracy of capillary blood for assessing vitamin A nutritional status among young children. Venous and capillary blood samples were simultaneously collected from 1366 healthy children under 7 years of age across 12 regions in China. Retinol was measured using high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). The agreement was assessed with Bland-Altman plot, Kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values. The sensitivity and specificity were evaluated using the ROC curve method. Venous and capillary retinol levels showed significant differences but were highly correlated with r of 0.93. Ordinary least squares regression was used to characterize (β = 0.913) and correct the systematic bias in capillary data (compared to paired venous). Thereafter, Bland-Altman analysis demonstrated that the mean bias of corrected capillary retinol compared to venous retinol was 0.01 (95%CI: -0.24, 0.25) μmol/L with no significant difference (p > 0.05). Corrected capillary retinol showed excellent performance for estimating vitamin A status when compared to venous retinol, with Kappa of 0.77-0.83, PABAK of 0.80-0.96, sensitivity of 0.86-0.91 and specificity of 0.87-0.98. Capillary HPLC-MS/MS method is therefore adequate for assessing vitamin A status of young children after correction for systematic bias

Bland–Altman plot · Kappa · Limits of agreement · Nuclear medicine · Retinol · Venous blood · Vitamin · Vitamin A deficiency · Antioxidant Activity and Oxidative Stress · Medicine · Reliability and Agreement in Measurement · Retinoids in leukemia and cellular processes · Gastroenterology · Internal Medicine