Validated stability-indicating RP-HPLC method for simultaneous estimation of candesartan and pioglitazone in human plasma
Dados Bibliográficos
| ID | 15792397 |
|---|---|
| Autores | Kola Hephzibah (SRM Institute of Science and Technology, autor correspondente), Sangeetha Shanmugasundaram (SRM Institute of Science and Technology, autor correspondente) |
| Ano | 2022 |
| Páginas | 10226-10239 |
| Data de publicação | 2022-09-13 |
| Peer Reviewed | Sim |
| Open Access | Sim |
| Tipo | ARTICLE |
| Periódico | International Journal of Health Sciences (JOURNAL) |
| Identificadores do periódico | ISSN: 2550-696X • E-ISSN: 2550-6978 |
| Editora | Universidad Tecnica de Manabi (PUBLISHER • EC) |
| DOI | 10.53730/ijhs.v6ns6.12718 |
| OpenAlex | W4295981673 |
| Idioma | EN |
| Referências citadas | 3 |
Objective: The focus of this study was to establish a simple, robust, and validated RP-column approach to analyze Candesartan and Pioglitazone in a bilayer tablet dosage form. To broaden patient compliance in conditions such as diabetes and hypertension, a combination of drugs is often prescribed. As a result, a bilayer tablet containing a fixed-dose combination of 8 mg of Candesartan and 15 mg of Pioglitazone was developed. Both drugs were analyzed and chromatographically separated using the liquid chromatography technique. Methodology: waters 2695 with a quaternary pump and Inertsil C18 column mobile phase as buffer: acetonitrile (60:40) and flow rate of 1ml per minute and detection at 220nm was used for performing Chromatography. Results: Internal standard, Candesartan, and Pioglitazone eluted with retention times of 2.208, 2.569, and 3.553 minutes, respectively. There was no interference found between the peaks. With a correlation value of 0.999, the approach is verified across a linear range of 0.5 μg/ml – 20.0μg/ml for Candesartan and 0.08 μg/ml – 3.2 μg/ml for Pioglitazone. 6 samples at LLOQ level were analyzed for accuracy and precision and found to be in limit and all the analytes were stable at 28 °C and -80 °C in human plasma
Analyte · Angiotensin II · Candesartan · Chromatography · Diabetes mellitus · High-performance liquid chromatography · Pioglitazone · Receptor · Resolution (logic · Type 2 diabetes · Analytical Chemistry and Chromatography · Analytical Methods in Pharmaceuticals · Chemistry · Computer Science · Medicine · Pesticide Residue Analysis and Safety · Biochemistry · Endocrinology
| Velocidade de citação | historical |
|---|---|
| Altamente citado | Não |