A Rapid and Sensitive stability indicating Rp-HPLC method development for the quantitative analysis of empagliflozin & linagliptin in bulk & synthetic mixture

An isocratic HPLC method was developed using, Shimadzu C18 column (250 mm × 4.6 mm, 5 μm) with an isocratic binary mobile phase consisting of Acetonitrile: Buffer in a ratio (80:20 v/v) pH 3.0 adjusted with Orthophosphoric acid and flow rate monitored at 0.80 ml/min. The UV detector was used for simultaneous analysis of two drugs at a common wavelength of 226 nm and each injection volume was 20 μl. The retention time for Empagliflozin and Linagliptin was found to be 3.714 min and 3.064 min, respectively. Empagliflozin and Linagliptin produce degradation products in acidic, alkaline, thermal, and UV stress. The result of the assay of Empagliflozin and Linagliptin shows that the degradation product does not interfere with the analytical procedure quantitively when these drugs are analyzed

Chromatography · Degradation (telecommunications · Empagliflozin · Forced degradation · High-performance liquid chromatography · Linagliptin · Quantitative analysis (chemistry · Reversed-phase chromatography · Chemistry · Diabetes Treatment and Management · Inflammatory mediators and NSAID effects · Peptidase Inhibition and Analysis