Validation of endotoxin‐core antibodies in dried blood spots as a measure of environmental enteropathy and intestinal permeability
Bibliographic Data
| ID | 19459978 |
|---|---|
| Authors | Morgan K Hoke (0000-0002-6752-6483, Department of Anthropology University of Pennsylvania Philadelphia Pennsylvania 19104, corresponding author), Kimberly A Mccabe (0000-0001-6178-9947, Department of Anthropology Northwestern University Evanston Illinois 60208), Aaron A Miller (0000-0003-2744-8916, Department of Anthropology Northwestern University Evanston Illinois 60208), T W Mcdade (0000-0001-5829-648X, Department of Anthropology Northwestern University Evanston Illinois 60208) |
| Year | 2018 |
| Volume | 30 |
| Issue | 4 |
| Pages | e23120-e23120 |
| Publication date | 2018-07-01 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | American Journal of Human Biology (JOURNAL) |
| Journal identifiers | ISSN: 1042-0533 • E-ISSN: 1520-6300 |
| Publisher | Wiley (PUBLISHER • GB) |
| DOI | 10.1002/ajhb.23120 |
| PMID | 29532544 |
| OpenAlex | W2791351234 |
| Language | EN |
| Citations received | 5 |
| References cited | 8 |
OBJECTIVE: To validate a method for measuring endotoxin-core antibodies (EndoCAb) from dried blood spots (DBS)-drops of capillary whole blood collected and dried on filter paper-as an indicator of environmental enteropathy (EE) in infancy and early childhood. METHODS: A commercially available enzyme immunoassay kit was adapted for use with DBS, with optimized steps for sample elution. Technical validation included analysis of linearity/recovery, precision and reliability, lower limit of detection, and correspondence between matched plasma and DBS samples. Validation in a field-based setting was implemented with samples from Peruvian infants (n = 82; age = 2-33 months) collected at two time points six months apart. RESULTS: = 0.93, P < .001). The lower limit of detection was found to be 0.01 GMU/mL. Interassay coefficient of variation (CV) was 10.9% and 8.06% for low and high controls, respectively. Mean intra-assay CVs were 3.22% and 1.83%, respectively. In a sample of Peruvian infants, EndoCAb levels increased with age as expected (P < .001). Age explained nearly 34.6% of the variance in EndoCAb across the sample. CONCLUSION: These findings demonstrate the validity and feasibility of measuring EndoCAb in remote field settings using minimally invasive DBS sampling
Animal science · Antibody · Biology · Blood sampling · Chromatography · Coefficient of variation · Confidence interval · Detection limit · Dried blood · Dried blood spot · Immunoassay · Biosimilars and Bioanalytical Methods · Chemistry · Infant Nutrition and Health · Medicine · Microscopic Colitis · Gastroenterology · Immunology · Internal Medicine
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| Unique citing works | 5 |
|---|---|
| Citations per year | 0.71 |
| Citation span | 2019 - 2025 (7) |
| Citation velocity | recent |
| Highly cited | No |
| Citation types | Neutral: 5 |