Association between stress severity in blood donors and lymphocyte radiosensitivity under low-dose computed tomography protocols
A pilot study using micronucleus assay
Bibliographic Data
| ID | 19480049 |
|---|---|
| Authors | Faina I Ingel (0000-0002-2262-6800), Sergey E Okhrimenko (0000-0002-8282-1798, City Clinical Hospital), Sergey A Ryzhkin (0000-0003-2595-353X, Kazan State Medical University), Lyudmila V Akhaltseva (0000-0002-3619-3858), Nadezhda A Urtseva (0000-0001-5031-2916), Elena K Krivtsova (0000-0002-5039-8980), Tatyana A Nikitina (0000-0003-0866-5990), Maria A Konyashkina (0000-0002-8319-1329), Victor A Gombolevskiy (0000-0003-1816-1315, Artificial Intelligence Research Institute) |
| Year | 2026 |
| Volume | 33 |
| Issue | 3 |
| Pages | 166-177 |
| Publication date | 2026-03-24 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | Ekologiya Cheloveka (Human Ecology (JOURNAL) |
| Journal identifiers | ISSN: 1728-0869 • E-ISSN: 2949-1444 |
| Publisher | ECO-Vector LLC (Publications) (PUBLISHER) |
| DOI | 10.17816/humeco697771 |
| OpenAlex | W7154718441 |
| Language | EN |
| References cited | 17 |
Background: Many studies on the effects of chemical genotoxicants in humans have demonstrated an association between the severity of stress and the observed manifestations of genome instability. A similar relationship has been reported following exposure of human blood samples to gamma radiation from 60Co at a dose of 1.0 Gy. However, the association between the severity of the stress response and the manifestation of genome instability under irradiation of blood samples using different low-dose computed tomography protocols has not been previously studied. Aim: The work aimed to test the hypothesis that the donor’s adaptive system influences the development of genome instability in blood lymphocytes under ex vivo irradiation using different low-dose computed tomography protocols. Methods: Nine apparently healthy volunteers (men and women) of different ages completed psychological questionnaires to assess stress severity and provided blood samples for irradiation under different low-dose computed tomography protocols. The blood samples were then cultured with cytochalasin B, followed by cytome analysis using the micronucleus assay. Results: An effect of stress severity on genome instability in blood lymphocytes (an increased proportion of asymmetric aneuploid trinucleated cells, as well as an increased proportion of rapidly dividing cells within the cell population spectrum) was observed only in cultures from donors experiencing maladaptive stress and only after irradiation at a dose of 0.82 mSv. Conclusion: The data obtained indicate that using of cytogenetic tests for comparative evaluation of radiation diagnostic technologies is currently appropriate. Furthermore, the developed experimental integrated approach may be used in future for assessing radiogenic risks ex vivo
Cytochalasin B · Ex vivo · Genome instability · Lymphocyte · Micronucleus · Micronucleus test · Population · Radiation therapy · Radiosensitivity · Carcinogens and Genotoxicity Assessment · Effects of Radiation Exposure · Radiation Dose and Imaging
| Citation velocity | historical |
|---|---|
| Highly cited | No |