Quantifying Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans Viability

Bibliographic Data

ID19528008
AuthorsAlexa Lindauer (University of Nevada, Reno), Tiffany May (University of Nevada, Reno), Gabriela Rios-Sotelo (0000-0002-5330-6295, University of Nevada, Reno), Ciara Sheets (University of Nevada, Reno), Jamie Voyles (0000-0002-0073-5790, University of Nevada, Reno, corresponding author)
Year2019
Volume16
Issue2
Pages346-350
Publication date2019-06-01
Open AccessYes
TypeARTICLE
VenueEcoHealth (JOURNAL)
Journal identifiersISSN: 1612-9202 • E-ISSN: 1612-9210
PublisherSpringer Science and Business Media LLC (PUBLISHER)
DOI10.1007/s10393-019-01414-6
PMID31124019
OpenAlexW2946009046
LanguageEN
References cited28

The disease chytridiomycosis is responsible for global amphibian declines. Chytridiomycosis is caused by Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), fungal pathogens with stationary and transmissible life stages. Establishing methods that quantify growth and survival of both life stages can facilitate research on the pathophysiology and disease ecology of these pathogens. We tested the efficacy of the MTT assay, a colorimetric test of cell viability, and found it to be a reliable method for quantifying the viability of Bd and Bsal stationary life stages. This method can provide insights into these pathogens' growth and reproduction to improve our understanding of chytridiomycosis

Amphibian · Animal ecology · Ascomycota · Biology · Chytridiomycosis · Chytridiomycota · Fungal disease · Zoology · Amphibian and Reptile Biology · Species Distribution and Climate Change · Turtle Biology and Conservation · Ecology · Microbiology

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