Genomic sequencing and neutralizing serological profiles during acute dengue infection
A 2017 cohort study in Nepal
Bibliographic Data
| ID | 19591371 |
|---|---|
| Authors | Sabita Prajapati (0000-0003-2140-8171, Tribhuvan University, corresponding author), Annie Elong Ngono (0000-0002-6741-3518, La Jolla Institute for Immunology, corresponding author), Melanie Mc Cauley (La Jolla Institute for Immunology, corresponding author), Julia Timis (La Jolla Institute for Immunology, corresponding author), Srijan Lal Shrestha (0000-0002-9390-8215, Tribhuvan University, corresponding author), Srijan Shrestha, Anup Bastola (0000-0003-3624-4720, Sukraraj Tropical & Infectious Disease Hospital, corresponding author), Shrawan Kumar Mandal (0000-0001-9475-2040, Sukraraj Tropical & Infectious Disease Hospital, corresponding author), Sanjay Yadav (0000-0002-1450-3202, Chitwan Medical College, corresponding author), Sanjay Ray Yadav, Rajindra Napit (0000-0002-3299-2986, Tribhuvan University, corresponding author), Meng Ling Moi (0000-0002-8103-6659, The University of Tokyo, corresponding author), Montarop Yamabhai (0000-0003-2674-2419, Suranaree University of Technology, corresponding author), October M Sessions (0000-0002-1370-2744, National University of Singapore, corresponding author), Sujan Shresta (0000-0003-2906-8474, La Jolla Institute for Immunology, corresponding author), Krishna Das Manandhar (0000-0002-6798-4935, Tribhuvan University, corresponding author) |
| Editors | Sukanta Chowdury |
| Year | 2024 |
| Volume | 4 |
| Issue | 11 |
| Pages | e0002966 |
| Publication date | 2024-11-13 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | PLOS Global Public Health (JOURNAL) |
| Journal identifiers | ISSN: 2767-3375 • E-ISSN: 2767-3375 |
| Publisher | Public Library of Science (PLoS) (PUBLISHER) |
| DOI | 10.1371/journal.pgph.0002966 |
| PMID | 39535994 |
| OpenAlex | W4404318700 |
| Language | EN |
| References cited | 43 |
Dengue virus (DENV) is a mosquito-borne flavivirus that poses a threat to nearly 50% of the global population. DENV has been endemic in Nepal since 2006; however, little is known about how DENV is evolving or the prevalence of anti-DENV immunity within the Nepalese population. To begin to address these gaps, we performed a serologic and genetic study of 49 patients from across Nepal who presented at central hospitals during the 2017 dengue season with suspected DENV infection. Of the 49 subjects assessed, 21 (43%) were positive for DENV NS1 antigen; of these; 5 were also anti-DENV IgM + IgG + ; 7 were DENV IgM + IgG − , 2 were IgM − IgG + , and 7 were IgM − IgG − by specific ELISAs. Seven of the 21 NS1 positive sera were RNA positive by RT-PCR (six DENV2, one DENV3), suggesting that DENV2 was the dominant serotype in our cohort. Whole-genome sequencing of two DENV2 isolates showed similarity with strains circulating in Singapore in 2016, and the envelope genes were also similar to strains circulating in India in 2017. DENV-neutralizing antibodies (nAbs) were present in 31 of 47 sera tested (66%); among these, 20, 24, 26, and 12 sera contained nAbs against DENV1, 2, 3, and 4 serotypes, respectively. Additionally, 27 (58%) samples had nAbs against multiple serotypes (2 or more). Serology analysis suggested that 12 (26%) and 19 (40%) of the 47 subjects were experiencing primary and secondary DENV infections, respectively. Collectively, our results provide evidence for current and/or past exposure to multiple DENV serotypes in our cohort. These data suggest that expanded local surveillance of circulating DENV genotypes and population immunity will be important to effectively manage and mitigate future dengue outbreaks in Nepal
Antibody · Biology · Dengue fever · Dengue vaccine · Dengue virus · Flavivirus · Population · Serology · Serotype · Virus · Malaria Research and Control · Medicine · Mosquito-borne diseases and control · Viral Infections and Vectors · Immunology · Virology
| Highly cited | No |
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