Disposable collection kit for rapid and reliable collection of saliva
Dados Bibliográficos
| AUTOR(ES) | |
|---|---|
| AFILIAÇÃO(ÕES) | Biomedical Engineering & Robotics Laboratory, Graduate School of Engineering, Iwate University 4‐3‐5 Ueda Morioka 020–8551 Japan, Graduate Course of Disability Science, University of Tsukuba 1‐1‐1 Tennodai Tsukuba 305–8577 Japan, Section of Oral and Maxillofacial Surgery, UCLA School of Dentistry, University of California 10833 Le Conte Avenue Los Angeles California 90095‐1668 |
| ANO | Não informado |
| TIPO | Artigo |
| DOI | 10.1002/ajhb.22696 |
| ADICIONADO EM | 2025-08-18 |
Resumo
ObjectivesTo describe and evaluate disposable saliva collection kit for rapid, reliable, and reproducible collection of saliva samples.MethodsThe saliva collection kit comprised of a saliva absorbent swab and an extractor unit was used to retrieve whole saliva samples from 10 subjects. The accuracy and precision of the extracted volumes (3, 10, and 30 μl) were compared to similar volumes drawn from control samples obtained by passive drool. Additionally, the impact of kit collection method on subsequent immunoassay results was verified by assessing salivary cortisol levels in the samples and comparing them to controls.ResultsThe recovered volumes for the whole saliva samples were 3.85 ± 0.28, 10.79 ± 0.95, and 31.18 ± 1.72 μl, respectively (CV = 8.76%) and 2.91 ± 0.19, 9.75 ± 0.43, and 29.64 ± 0.91 μl, respectively, (CV = 6.36%) for the controls. There was a close correspondence between the salivary cortisol levels from the saliva samples obtained by the collection kit and the controls (R2 > 0.96).ConclusionsThe disposable saliva collection kit allows accurate and repeatable collection of fixed amounts of whole saliva and does not interfere with subsequent measurements of salivary cortisol. The simple collection process, lack of elaborate specimen recovery steps, and the short turnaround time (<3 min) should render the kit attractive to test subjects and researchers alike. Am. J. Hum. Biol. 27:720–723, 2015. © 2015 The Authors American Journal of Human Biology Published by Wiley Periodicals, Inc.