Evaluation of the Anti-Mycobacterial, Anti-Oxidative and Anti-Proliferative Activities of Rubia Cordifolia Ethanolic Leaf Extract Sub-Fractions in Human Lymphocytes and Neutrophils
Bibliographic Data
| ID | 20345507 |
|---|---|
| Authors | Makgatho ME, Winston Nxumalo (0000-0002-8310-4520, University of Limpopo), Nxumalo W, Ndaba E, E Ndaba (University of Limpopo), C Masilo, F Tsindane (University of Limpopo), Ml Sedibane |
| Year | 2017 |
| Volume | 14 |
| Issue | 5 |
| Pages | 181-187 |
| Publication date | 2017-10-01 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) (PUBLISHER) |
| DOI | 10.21010/ajtcam.v14i5.21 |
| OpenAlex | W2761420837 |
| Language | EN |
| References cited | 3 |
Background. Rubia cordifolia has been used to treat various diseases and exhibits antimicrobial, anti-inflammatory, analgesic and antidiarrheal activities. The plant has not been investigated for its anti-mycobacterial activity against virulent tuberculosis strains as well as cytotoxic and anti-oxidant activities in mononuclear cells and neutrophils. Methods. Ethanolic and dichloromethane leaf extract fractions of Rubia cordifolia (0.2- 125μg/ml) were screened for anti-mycobacterial activity using a fluorescent microplate assay. The anti-oxidant activity of the fractions was tested using the radical scavenging assay, while their cytotoxicity to lymphocytes was measured using the WST-8 assay. Reactive oxygen species production by neutrophils exposed to fractions was tested using Cell MeterTM Fluorimetric ROS Assay kit. Results. The ethanolic leaf fractions exhibited overall superior anti-mycobacterial activity than the dichloromethane group and were further screened for anti-oxidant and cytotoxic activities. Ethanolic sub-fractions K2F-3.2, K2F-3.3 and K2F-3.4 showed significant antioxidant activities at concentration of 50 μg/ml to 200 μg/ml and higher in both the cell free-based radical scavenging activity and total reactive oxygen species production assays. For lymphocytes, the test agents showed anti-proliferative activity at 25 μg/ml to 200 μg/ml for sub-fraction K2F-3.3B and 50 μg/ml to 200 μg/ml for agents K2F-3.2 and K2F-3.4. Conclusion. The current study is the first to record the in vitro anti-mycobacterial, anti-oxidant and cytotoxic activities of Rubia cordifolia plant leaf extract sub-fractions using the given experimental setups and further research activities to identify the bioactive components are to be pursued
Anti-inflammatory · Antimicrobial · Antioxidant · Biology · Cytotoxicity · Flavonoid · In vitro · Reactive oxygen species · Tinospora cordifolia · Traditional medicine · Chemistry · Medicine · Morinda citrifolia extract uses · Pharmacological Effects of Natural Compounds · Biochemistry · Pharmacology
Medicinal plants used for the treatment of tuberculosis by Bapedi traditional healers in three districts of the Limpopo province, South Africa
In vitro inhibition of drug-resistant and drug-sensitive strains of Mycobacterium tuberculosis by ethnobotanically selected South African plants
Antimycobacterial and cytotoxic activity of selected medicinal plant extracts
| Highly cited | No |
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