Effects of Zamzam Water and Methadone on the Expression of Mu-Opioid Receptor-1 Gene in Morphine-Dependent Rats After Chronic Morphine Administration
Bibliographic Data
| ID | 20345778 |
|---|---|
| Authors | Shariff Halim (0000-0003-3792-9879, Sultan Zainal Abidin University), Nasir Mohamad (corresponding author), Nor Hidayah Abu Bakar (0000-0003-0721-5090), Rohayah Husain (0000-0002-1066-5466), Khairi Che Mat (0000-0001-9837-0138), Syed Hadzrullathfi Syed Omar (0000-0003-0315-282X), Mohd Shahril Othman, Nur Atikah Muhammad, P M Ridzuan (Sultan Zainal Abidin University), Nor Zidah Ahmad |
| Year | 2018 |
| Volume | 15 |
| Issue | 2 |
| Pages | 19-25 |
| Publication date | 2018-03-23 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) (PUBLISHER) |
| DOI | 10.21010/ajtcam.v15i2.3 |
| OpenAlex | W4252088502 |
| Language | EN |
| References cited | 1 |
Background: Sodium ion is an essential ion that is implicated in many physiological functions. Recently, sodium ion was reported to facilitate the activation of Mu-Opioid Receptor (MOR) by binding at the allosteric site of the MOR. Zamzam water is water originated from Mecca. Couple of studies proved that Zamzam water has the therapeutic effect owing to its mineral. In this study, we want to determine the ion concentration of Zamzam water and then to investigate the effects of Zamzam water and co-treatment with methadone on the regulation of MOR-1gene after chronic morphine administration. Materials and Methods: Zamzam water, tap water and normal mineral water were analyzed using Ion chromatography. Meanwhile, in animal study, 50 male Sprague Dawley rats were randomly divided into five groups. All group of rat were made dependence on morphine using intraperitoneal injection except for normal group. Morphine dependent rats then were treated with methadone, Zamzam water and co-treatment methadone with Zamzam water for thirty days, respectively. The Ventral Tegmental Area (VTA) of rat's brain was dissected and subjected to real-time quantitative RT-PCR to determine the regulation of MOR-1 gene expression. The obtained data were analyzed using SPSS v.11 software, and one-way ANOVA followed by Tukey's Post-test
Anesthesia · Chronic pain · Methadone · Morphine · Opioid · Receptor · μ-opioid receptor · Diet and metabolism studies · Medicine · Internal Medicine · Pharmacology
| Highly cited | No |
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