Withaferin a Induces Apoptosis in Rat C6 Glioma Cells Through Regulating Nf- Κb Nuclear Translocation and Activation of Caspase Cascade
Bibliographic Data
| ID | 20345895 |
|---|---|
| Authors | Wei-chen Hou, Weichen Hou (Jilin University), Xiao-hui Miao, Xiaohui Miao (Changchun University of Chinese Medicine), Lian-jun Ma, Lianjun Ma (Union Hospital), Xiao-xue Bai, Xiaoxue Bai (0000-0002-6471-1819, Jilin University), Qun Liu (0000-0002-7000-1792, Jilin University, corresponding author), Lei Song (0000-0001-6166-6189, Jilin University) |
| Year | 2017 |
| Volume | 14 |
| Issue | 2 |
| Pages | 319-324 |
| Publication date | 2017-01-13 |
| Peer Reviewed | Yes |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) (PUBLISHER) |
| DOI | 10.21010/ajtcam.v14i2.33 |
| PMID | 28573248 |
| OpenAlex | W2574818002 |
| Language | EN |
| Citations received | 1 |
| References cited | 2 |
Background: The demand for the chemopreventive drug from the plant source is increasing in recent times, owing to its various biological activities without any adverse effect. The intention of this current study was to examine the antiglioma effect of Withaferin A (WFA) on C6 glioma cell line model. Materials and Methods: C6 glioma cells were administrated with different concentration of WFA (50, 100, 200 and 500 μg/mL) and DMSO (control) group to examine its anti-proliferative, anti-inflammatory and pro-apoptotic activities. Results: Treatment with WFA showed a significant decline in the glioma cell count in a dose-dependent manner and thus proving its anti-proliferative effect. Similarly, inflammatory markers were also substantially lowered upon treatment with different concentration of WFA. However, DNA fragmentation and apoptotic markers like Caspase-3 and 9 were concomitantly enhanced after co-cultured with different concentration of WFA and thus exhibiting its cytotoxicity efficacy. Furthermore, the protein expression of Bcl2 and Bax were markedly downregulated and upregulated respectively; upon treatment with WFA on C6 glioma cells. Conclusion: The outcome of this study evidently demonstrates that C6 glioma cells co-cultured with increased concentration of WFA, showed an anti-proliferative, anti-inflammatory and pro-apoptotic effect in a dose-dependent fashion
Apoptosis · Biology · Caspase 3 · Cell culture · Cell growth · Cytotoxicity · DNA fragmentation · Downregulation and upregulation · Glioma · In vitro · NF-κB · Pathology · Programmed cell death · Withaferin A · Andrographolide Research and Applications · Chemistry · Medicine · Pharmacology and Nanomedicine Research · Phytochemicals and Medicinal Plants · Biochemistry · Cancer Research · Genetics · Pharmacology
| Unique citing works | 1 |
|---|---|
| Citations per year | 0,17 |
| Citation span | 2020 - 2020 (1) |
| Citation velocity | historical |
| Highly cited | No |
| Citation types | Neutral: 1 |