Protective Effects of Cistanches Herba Aqueous Extract on Cisplatin-Induced Premature Ovarian Failure in Mice
Bibliographic Data
| ID | 20346245 |
|---|---|
| Authors | Pengyu Pan (0000-0001-8694-5483, Liaoning University of Traditional Chinese Medicine), Ying Wang (0000-0003-3126-1134, Liaoning University of Traditional Chinese Medicine), Xue Leng (0000-0001-6173-5981, Liaoning University of Traditional Chinese Medicine), Jinfang Deng (0000-0001-7910-167X, Jingzhou Central Hospital), Chun Wang (0009-0002-7869-0892, Liaoning University of Traditional Chinese Medicine) |
| Year | 2017 |
| Volume | 14 |
| Issue | 6 |
| Pages | 90-101 |
| Publication date | 2017-11-15 |
| Open Access | Yes |
| Type | ARTICLE |
| Venue | African Journal of Traditional Complementary and Alternative Medicines (JOURNAL) |
| Journal identifiers | ISSN: 0189-6016 • E-ISSN: 2505-0044 |
| Publisher | African Traditional Herbal Medicine Supporters Initiative (ATHMSI) (PUBLISHER) |
| DOI | 10.21010/ajtcam.v14i6.10 |
| OpenAlex | W2768780240 |
| Language | EN |
| References cited | 2 |
Background: Chemotherapeutic treatment of premenopausal women has been linked to premature ovarian failure (POF). Cistanches Herba (CH) is a commonly used male impotence and female infertility treatment in China; however, whether CH protects ovaries from chemotherapeutic drug-induced POF remains unclear. In this study, we investigated the protective effects of CH in a mouse model of chemotherapeutic drug-induced POF. Materials and Methods: We administered low- and high-concentration CH to cisplatin-induced POF mice for 2 weeks and determined body and ovarian weights, as well as serum follicle-stimulating hormone (FSH) and estradiol concentrations, to evaluate ovarian function. In addition, we evaluated the protective mechanisms of CH by detecting the levels of apoptosis-related proteins and evaluating markers of mitochondrial function. Results: In POF mice, we observed reduced body and ovarian weights; elevated serum FSH and attenuated estradiol concentrations; apoptosis of ovarian granulosa with concomitant changes in apoptosis-related proteins (including caspase-3, poly adenosine diphosphate-ribose polymerase, Bcl-2, and Bax); and mitochondrial dysfunction, such as a reduction in mitochondrial numbers, destruction of ultrastructural morphology, decrease in ATPase activity, and decreases in mitochondrial membrane potential and mitofusin-2 (a mitochondria dynamin-like GTPase). Significantly, CH reversed, to an extent, functional and morphologic injuries and ovarian tissue apoptosis by up-regulating the level of Mfn2 and the ratio of Bcl-2/Bax. Furthermore, CH reduced cisplatin-induced mitochondrial dysfunction in ovarian tissues. Conclusion: The present findings showed that CH inhibited cisplatin-induced POF through interactions between Mfn2 and Bcl-2/Bax proteins and, possibly, by up-regulation of Mfn2 expression. Ultimately, CH protects ovarian tissues from cisplatin-induced apoptosis
Aqueous solution · Biology · Chemotherapy · Cisplatin · Premature ovarian failure · Chemistry · Medicine · Ovarian function and disorders · Phytochemical and Pharmacological Studies · Reproductive Biology and Fertility · Internal Medicine · Pharmacology
| Highly cited | No |
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